Scanning dynamics of the mammalian ribosome during translation initiation (A15*)

大多数真核生物mRNAs的翻译都是通过扫描机制开始的。小的核糖体亚基被招募到mRNA5‘端，然后向AUG起始密码子的3’方向迁移。细胞已经发展出多种机制来调节扫描过程，从而塑造他们的蛋白质组。监管不当可能会导致癌症和病毒感染等疾病。我们将研究使用单分子Förster共振能量转移(SmFRET)和荧光全内反射(TIRF)显微镜进行扫描。我们的工作将建立真核生物翻译过程中基因表达的功能和调控机制。

Translation of most eukaryotic mRNAs starts by a scanning mechanism. The small ribosomal subunit is recruited to the mRNA 5’ end and then migrates in 3’ direction towards the AUG start codon. Cells have developed multiple mechanisms to regulate the scanning process and thus shape their proteome. Misregulation can lead to diseases such as cancer and viral infections. We will study scanning using single molecule Förster Resonance Energy Transfer (smFRET) and Total Internal Reflection of Fluorescence (TIRF) microscopy. Our work will establish the mechanisms of function and regulation of gene expression during translation in eukaryotes.