Dialogue(s) for fertility: deciphering spermatozoa-oviduct interactions and their implications for artificial insemination in dairy cattle

在受孕前后，特定时间的荷尔蒙变化发生在输卵管中，调节精子与母体的相互作用，精子存活和受精。破译这些荷尔蒙驱动的相互作用将有助于改善人工授精结果，这是乳制品行业的主要利害关系之一。因此，为期3年的对话项目的目标是：1)更好地描述体内输卵管分泌体的特征；2)将体内输卵管环境转化为精确的体外模型，以真实地模拟发情周期的不同阶段；3)使用该模型来破译精子-输卵管对话的调节以及这些相互作用对精子生理的影响；4)确定精子-输卵管相互作用的机制和可用于优化牛授精条件的分子。德国的Jennifer Schoen和法国的Marie Saint-Dizier将协调该项目。德国申请者是位于Dummerstorf的莱布尼茨农场动物生物学研究所(FBN)生殖生物学研究所的生殖细胞生物学部门：该团队在建立和使用高度分化的输卵管细胞培养模型方面拥有丰富的专业知识。为了这个项目，他们与初级研究小组血糖生物学和FBN的细胞计数核心设施合作。法国申请者是位于努齐利INRA Val-de-Loire中心的UMR 85 INRA/CNRS/IFCE/University Tour生殖和行为生理学(PRC)。该单位的细胞相互作用和受精(ICF)团队在分析牛输卵管生理调节方面拥有丰富的专业知识，并将与生物分子综合分析平台(PAIB)合作开展这一项目。该对话项目阐明了对牛的生殖健康至关重要的细胞和分子机制，牛是一种具有重要经济意义的农场动物。此外，它还为开发新工具以优化该物种的繁殖管理和遗传育种奠定了基础。

Around the time of conception, time-specific hormonal changes occur in the oviduct, modulating sperm-maternal interactions, sperm survival and fertilization. Deciphering these hormonal driven interactions will be useful to improve artificial insemination outcome, one major stake in dairy industry. Thus, the objectives of the 3-year DIALOG project are to: 1) better characterize the oviductal secretome in vivo; 2) translate the in vivo oviductal milieu into a refined in vitro model to faithfully mimic the different phases of the estrous cycle; 3) use this model to decipher the regulation of the sperm-oviduct dialogue and the effects of these interactions on sperm physiology; 4) identify mechanisms of sperm-oviduct interactions and molecules that could be used to optimize inseminating conditions in cattle. Jennifer Schoen in Germany and Marie Saint-Dizier in France will coordinate the project. The German applicant is the Reproductive Cell Biology Unit of the Institute of Reproductive Biology at the Leibniz Institute for Farm Animal Biology (FBN) located in Dummerstorf: this team has a strong expertise in establishing and using highly differentiated oviduct cell culture models. For this project they collaborate with the Junior Research Group Glycobiology and the Cytometry Core Facility of the FBN. The French applicant is the UMR 85 INRA/CNRS/IFCE/Université de Tours Physiology of Reproduction and Behaviors (PRC) located in the Centre INRA Val-de-Loire (Nouzilly). The Cellular Interactions and Fertility (ICF) team in this unit has a strong expertise in analyzing the regulation of the bovine oviduct physiology and will work on this project in collaboration with the Platform for Integrative Analysis of Biomolecules (PAIB). The DIALOG project elucidates cellular and molecular mechanisms crucial for reproductive fitness in cattle, a farm animal of major economic relevance. It furthermore builds the basis for the development of new tools to optimize reproduction management and genetic breeding in this species.