Development of the new combination therapy used by Super selective extra coporal local hyperthermia.

经导管动脉栓塞术已被广泛应用于不可切除的肝肿瘤的保守治疗。然而，由于TAE不完整和侧支动脉发育导致肿瘤血运重建，其疗效仍不理想。提出了一种新的非手术性肝肿瘤的治疗方法，即用含有金属颗粒或磁流体的玉米蛋白在TAE后进行射频微波辐照的体外局部热疗，提高局部热疗的准确性。这项实验研究是为了开发合适的栓塞剂，并评估这种联合治疗的协同细胞毒性作用。永久性栓塞物质选择玉米蛋白和钴磁流体。采用不锈钢材料中的金属颗粒作为热介质。体外热疗采用2450 MHZ微波辐射。这项工作的目的如下：1)玉米蛋白作为永久性TAE剂和金属颗粒包衣物质的质量；2)在金属颗粒栓塞区域带来局部高温条件的可能性；3)该方法对荷瘤日本大白兔的细胞毒性作用。玉米蛋白在碱性溶液中溶解，在低温条件下能顺利凝固，但在加温条件下也不可逆。此外，未检测到炎症反应。但是，磁流体引起了炎症反应。由于放射性金属颗粒和磁性液体的存在，TAE的情况很容易检测，这也为热疗疗效的随访提供了优势。在明胶模体中含金属颗粒和不含金属颗粒的部位及肝组织中进行微波辐射后，观察到明显的温度梯度。在荷瘤动物的实验研究中发现，术后肿瘤受累坏死区有增加的趋势。热疗的细胞毒性作用是基于正常血管与肿瘤血管的功能差异以及热疗后正常细胞与肿瘤细胞的敏感性差异。因此，我们采用永久性TAE联合热疗治疗含肿瘤累及区金属颗粒的新方法，可能具有该区域所证实的强烈的细胞毒作用，可能具有本实验所证实的强烈的细胞毒作用。少

The transcatheter arterial embolization (TAE) has been widely used as the conservative therapy for non-resectable liver tumor. However, due to the revascuralization to the tumor caused by the imcomplete TAE and the developing collateral arteries, its efficacy has been still unsatisfied. The new therapeutic method for non-operative liver tumoe was designed to apply extra-corporal local hyperthermia by the microwave of RF irradiation after TAE by Zein contained with metal particles or magnetic fluid which enhance the localized hyperthermic errect.This experimental study was undertaken to develope the appropriate embolic agents for this purpose and to assess the synergic cytotoxic effect to this combination therapy.Zein which belongs to prolamin and cobalt magnetic fluid were chosed as the permanent embolic substances. Metal particles which made from Tital of Stainless material were used as hyperthermic mediating agent. The radiation of 2450 MHZ microwave was employed for the extra-corpor … More al local hyperthermia. This work was done to examine as followd; 1) the quality of Zein for the aims of permanent TAE agent and metal particle coating substance, 2) the possibility to bring local hyperthermic condition on the metal particle embolized region, 3) the cytotoxic effect of this procedure using tumor-bearing Japanese white rabbits.Zein is dissolved in alkaline solution and coagulate smoothly by the lowering temperature, but that is irreversible even in the rewaming condition. Furthermor, no inflammatory reaction could be detected. But, the magnetic fluid caused inflammatory reactions. It is easy to detect the condition of TAE due to the radiopague metal particles and magnetic fuluid which also provide the advantage to follow-up the efficacy of the hyperthermic treatment. The remarkable temperature gradient was observed after the mictowave radiation between the part with and without metal particles in gelatin phantom or in liver tissue. The tendency of the increase of necrotized tumor involved region was detected in the experimental study of tumor bearing animals after this procedure.Cytotoxic effect by the hyperthermia is based on the functinal difference between normal vessels and tumor vessels and the differnce of sensibivity between normal cells and tumor cells of which the heat errect. Therefor, out new procedure which employs permanent TAE combined with hyperthermia in metal particles contained tumor involved region, might have the intense cytotoxic effect which proved by this region, might have th intense cytotoxic effect which proved by this experiment. Less