Studies on the inhibitors from plant pathogens produced by soil germs

1.铜绿假单胞菌S-7是从紫田土壤中分离到的一株能抑制尖孢镰刀菌FA-3生长的菌株。土壤中的细菌用甲醇提取，提取液在薄层层析板上展开，薄层层析板上铺有培养的F。尖孢菌属和具小窝的。含有生长抑制剂的TLC条带通过琼脂上的清晰区域显示。从透明带中分离出三种抑制剂，分别鉴定为半胡椒碱、氯萘和吩嗪-1-羧酸。抑制F.尖孢霉（oxysporum），浓度分别为100、200和200 μ g/ml。北海道农业实验站正在开发这些抑制活性的实际应用。从田间土壤中分离到的洋葱假单胞菌B-17也对F.尖孢菌属该菌的最低浓度为3.13 μ g/ml。该抑制剂被鉴定为吡咯尼嗪。另一菌株RB 425对立枯丝核菌和大丽轮枝菌有较强的抑制作用，其抑制剂为假聚糖类，2-庚烯基-3-甲基-4-羟基喹啉和2-壬烯基-3-甲基-4-羟基喹啉。对根癌农杆菌、密歇根棒杆菌、胡萝卜软腐欧文氏菌、青枯假单胞菌和黄单胞菌也有抑制作用。壬烯基假单胞菌具有广泛的活性，尤其是对乙酰氨基酚的活性。灰盖乳牛肝菌具有抑制脂肪过氧化物生成的活性，并分离得到了抑制剂。用离体肝细胞在ADP和NADPH存在下的脂肪酶过氧化反应评价活性，用TBA法测定过氧化脂肪。通过光谱分析确定了新合成的缓蚀剂的结构为1-乙酰氧基-2，4-二羟基-6-香叶基香叶基苯。

1. Pseudomonas aeruginosa S-7, separated from the soil of azuki-field inhibited the growth of Fusarium oxysporum FA-3. The germ from the soil was extracted with methanol and the extracts were developed on TLC which was laid on the agar with cultivated F. oxysporum and icnubated. The TLC bands containing growth inhibitors were showed by clear zones on the agar. Three inhibitors were isolated from the clear zones and they were identified with hemipiocyanine, chlororaphine and phenazine-1-carboxylic acid. They inhibited F. oxysporum with the concentration 100, 200 and 200mug/ml respectively. Practical application of these inhibition activity was now developed by the Agricultural Experimental Station, Hokkaido.2. Pseudomonas cepacia B-17 separated from the soil of the field also inhibited F. Oxysporum. The germ by the minimum concentration of 3.13mug/ml. The inhibitor was identified with pyrrolnitrine. Another strain RB425 strongly inhibited Rhizoctonia solani and Verticillium dahlial and inhibitors were assigned to pseudans, 2-hepteny1-3-methy1-4-quinolinol and 2-noneny1-3-methy1-4-quinolinol. They inhibited Agrobacterium tumefaciens, Corynebacterium michigenese, Erwinia carotovora, Pseudomonas solanocearum and Xanthomonas oryzae, too. The nonenyl pseudane showed broad activity, especially.3. Suillus grevillei showed the activity of inhibiting from the production of fat peroxide and the inhibitor was isolated. Activity was evaluated by the enzymatic peroxidation of fat under existence of ADP and NADPH using separated liver cell in vitro and peroxygenated fat was measured by TBA method. The structure of newly obtained inhibitor was determined as 1-acetoxy-2,4-dihydroxy-6-geranyl geranyl benzene by mainly spectroscopic methods.