Network Regulation of Expression and Activity of Binding Protein-Dependent ABC-Sugar Transport Systems of Thermus thermophilus

嗜热栖热菌是一种嗜热细菌，最有可能代表当今革兰氏阳性菌和阴性菌之间的进化中间体（15）。在糖转运系统方面，它依赖于ABC转运蛋白，但与古细菌一样，它尚未获得糖磷酸转移酶系统（PTS）。我们将继续研究MalK的功能，MalK是T.嗜热菌我们将分析MalK选择性的机制，在服务于不同的ABC转运依赖于运输活动的状态。我们建议，在没有分解代谢物阻遏的情况下，碳利用率的选择是通过控制MalK与各自的渗透酶的结合以及MalK的调节结构域与参与糖代谢的蛋白质的特异性相互作用来介导的。反之亦然，我们将遵循MalK的可能性，取决于其参与运输将影响代谢途径。在这里，我们将重点关注糖原的合成和降解。

Thermus thermophilus is a thermophilic bacterium that most likely represents an evolutionary intermediate between today's Gram positive and negative bacteria (15). In respect to sugar transport systems it relies on ABC transporters but as in archaea it has not yet aquired the sugar phosphotransferase system (PTS). We will proceed to study the function of MalK, the common transport ATPase for three ABC systems in T. thermophilus. We will analyse the mechanism of MalK-selectivity in serving the different ABC transporters dependent on the status of transport activity. We propose that in absence of catabolite repression the selection of carbon utilization is mediated by controlling the binding of MalK to the respective permeases and by the specific interaction of the regulatory domain of MalK with proteins involved in sugar metabolism. Vice versa we will follow the possibility that MalK, depending on its engagement in transport will affect metabolic pathways. Here we will focus on glycogen synthesis and degradation.