Dissecting the molecular interplay between tumor cells and stroma in primary live cancer (B08#)

目前尚不清楚是哪些改变驱动了肿瘤表型或决定了其生物学行为。基于严格的形态学评估，我们选择了显示少量或广泛肿瘤基质的人类原发性肝癌亚组，并进行全基因组外显子组和转录组分析，以缩小潜在影响肿瘤基质进化和肿瘤细胞表型的候选基因。这些将使用马赛克小鼠模型进行功能验证和表征。这种综合方法将提高我们对肿瘤细胞-间质相互作用的认识，并可能有助于确定可能特别受益于针对肿瘤间质的治疗干预的患者。

It remains elusive which alterations are driving the tumor phenotype or determine its biological behavior. Based on a stringent morphological evaluation we selected subgroups of human primary liver cancer showing either minimal or extensive tumor stroma and perform genome-wide exomic and transcriptomic profiling to narrow in on candidate genes potentially affecting the evolution of the tumor stroma and the phenotype of the tumor cells. These will be functionally validated and characterized using mosaic mouse models. This integrative approach will improve our knowledge on the tumor cell-stroma interaction and may help to identify patients that may especially benefit from therapeutic interventions targeting the tumor stroma.