Quantitative interactomics of specific types of DNA damage (01)

在本提案中，我们将使用定量高分辨率质谱法来鉴定在不同细胞类型和物种中重新募集到一系列定义的DNA病变的蛋白质，例如8-oxo-dG，环丁烷嘧啶二聚体和链内顺铂-DNA加合物。假定的新候选者将通过建立的生化和细胞生物学技术进一步表征。通过这种方法，我们的目标是识别参与这些DNA损伤修复的复杂蛋白质网络，并揭示从酵母到人类的生物中DNA损伤修复的异同。

In this proposal, we will use quantitative high-resolution mass spectrometry to identify proteins re-cruited to a range of defined DNA lesions, such as 8-oxo-dG, cyclobutane pyrimidine dimers and intrastrand cisplatin-DNA adducts, in different cell types and species. Putative new candidates will be further characterised by established biochemical and cell biology techniques. With this approach, we aim to identify complex networks of proteins involved in repair of these DNA lesions and uncover similarities and differences in DNA damage repair in organisms ranging from yeast to humans.