Co-transcriptional repair and transcriptional bypass of bulky DNA adducts (04)

该项目的目标是了解转录基因内DNA损伤对受影响基因活性的影响。为此，我们将在选定的位置将一系列生理上相关的DNA加合物纳入转录模板，并研究细胞中这些病变的转录旁路的效率和准确性。此外，我们将讨论转录DNA中结构不同的DNA损伤的识别模式，以便通过人类核苷酸切除修复途径的两个主要分支确定检测和去除受损核苷酸的决定因素。

The goal of this project is to understand the consequences of DNA lesions within a transcribed gene for the activity of the affected gene. To this end, we will incorporate a range of physiologically rele-vant DNA adducts at selected positions into a transcription template and investigate the efficiencies and accuracies of transcriptional bypass of these lesions in cells. In addition, we will address the modes of recognition of structurally distinct DNA lesions in transcribed DNA in order to identify the determinants for detection and removal of damaged nucleotides by the two major branches of the human nucleotide excision repair pathway.