High resolution hybrid mass spectrometer

该应用程序的目标是在Freie Universität柏林新获得的高分辨率质谱仪。该仪器计划用于蛋白质组学研究，鉴定翻译后修饰，并通过天然质谱法研究完整蛋白质或蛋白质复合物。作为现有biosuperamol设施的一部分，该光谱仪计划安装在生物/化学/药学系，并应满足各个研究小组的不同要求。计划中的项目包括从鉴定复杂混合物中蛋白质网络的典型原生学应用到与蛋白质复合物形成的结构和分子机制相关的问题。将要研究的生物系统也有不同的起源，例如，包括细菌中蛋白质组和相互作用组在变化的环境条件下的变化问题，以及在刺激神经或免疫系统时要求蛋白质修饰的问题。

The goal of this application is the new acquisition of a high resolution mass spectrometer at Freie Universität Berlin. The instrument is planned to be used for proteomic investigations, for the identification of post-translational modifications and for the investigation of intact proteins or protein complexes by native mass spectrometry. As part of the existing BioSupraMol facility the spectrometer is intended to be installed at the Department of Biology/Chemistry/Pharmacy and should serve the diverse requirements of the individual research groups. The intended projects reach from typical protoemic applications for the identification of protein networks in complex mixtures to questions related to structure and molecular mechanism underlying protein complex formation. The biological systems that will be interrogated are also of diverse origin and for example comprise questions of the change of proteomes and interactomes in bacteria upon changing environmental conditions to questions that ask for modifications of proteins upon stimulation of the nervous or immune system.