Mode of action of cell wall biosynthesis inhibitors in Wolbachia spp. (A08)

我们将阐明专性细胞内沃尔巴克氏菌中脂质II周期和细胞分裂之间的联系，沃尔巴克氏菌是一种被认为没有细胞壁的革兰氏阴性α-蛋白细菌属。利用磷霉素，将在细胞和分子水平上研究抑制Mura对细胞分裂的影响。我们将利用转录组学和蛋白质组学来阐明磷霉素是如何从宿主细胞中耗尽这些内生菌的，以及它们如何应对脂质II循环的阻断，包括它的循环。利用标记的前体，沃尔巴克氏菌将被探测到一种基本的肽聚糖，并将研究脂II在与细胞分裂机制(如MreB或FtsZ)相互作用或协调中的作用。

We will elucidate the connection between the lipid II cycle and cell division in the obligate intracellular Wolbachia, a genus of Gram-negative alpha-proteobacteria thought to have no cell wall. Using fosfomycin, the impact of inhibiting MurA on cell division will be studied at the cellular and molecular levels. We will employ transcriptomics and proteomics to clarify how fosfomycin depletes these endobacteria from host cells and how they respond to blocking of the lipid II cycle, including its recycling. Using labeled precursors, Wolbachia will be probed for a rudimentary peptidoglycan and the role of lipid II in interacting with or coordinating the cell division machinery (e.g. MreB or FtsZ) will be investigated.