Real time PCR and nanoparticle diagnostic facilities for high-throughput quantitative analysis of genomic structure and gene expression

现代分子工具导致了基因组计划的爆炸性增长和生物学所有领域的统一。这类研究最基本的需要是获得改进的技术，以检测区分不同基因的DNA指纹，并确定哪些基因在不同情况下“开启”或“关闭”。实时聚合酶链式反应技术是昆士兰大学(UQ)和南十字大学(SCU)在1996年利用ARC资金率先开发的，现在是这项研究的大部分技术的选择。该项目将为实时聚合酶链式反应提供高通量设备，并将开发互补的高通量“纳米颗粒”DNA基因分型技术，应用于医药和农业。

Modern molecular tools have lead to an explosion in genome projects and unification of all areas of biology. The most basic need for such research is access to improving technologies for detecting DNA fingerprints that distinguish genetically-diverse genes, and determining which genes are "switched on" or 'off' in various situations. Real time PCR technology, pioneered by The University of Queensland (UQ) and Southern Cross University (SCU) using ARC funding in 1996, is now the technology of choice for much of this research. This project will provide high-throughput equipment for real time PCR, and will develop complementary high-throughput "nanoparticle" DNA genotyping technologies, with applications to medicine and agriculture.