Vascularized neuromuscular constructs on PCL-collagen I-PEO-nanoscaffolds

容积性肌肉丢失是关键，因为它超过了骨骼肌组织的自然再生能力。骨骼肌组织工程可能是一种治疗方案，而不必牺牲其他功能肌肉。为此，需要合适的细胞和足够的基质，代表骨骼肌的分层和复杂的三维结构。此外，体积3D组织结构需要充分的血管形成。对于功能性肌肉的生成，新生组织的神经支配也是必不可少的。本研究的目的是通过将原代成肌细胞、脂肪组织来源的干细胞(ADSC)和雪旺细胞共同培养在排列的PCL-胶原I-纳米纤维上来生成骨骼肌组织。细胞将被肌源性分化，3D结构将在体内植入动静脉(AV)环模型，并整合运动神经，即所谓的EPI环模型，以实现神经化。通过这些手段，我们的目标是创造血管化和功能性的骨骼肌组织。

Volumetric muscle loss is critical since it exceeds the natural regeneration capacity of skeletal muscle tissue.Tissue engineering of skeletal muscle could be a treatment option without having to sacrifice other functional muscles. For this purpose, suitable cells as well as an adequate matrix, representing the hierarchical and complex 3D structure of skeletal muscle, are needed. Furthermore, volumetric 3D tissue constructs necessitate adequate vascularization. For the generation of functional muscle, innervation of the neotissue is also necessary.Aim of this study is the generation of skeletal muscle tissue by co-cultivating primary myoblasts, adipose tissue-derived stem cells (ADSC), and schwann cells on aligned PCL-collagen I-nanofibers. Cells will be myogenically differentiated and the 3D constructs will be implanted in vivo into an arteriovenous (AV-) loop model with integration of a motoric nerve, the so-called EPI-loop model, to enable neurotisation. By these means, we aim to create vascularized and functional skeletal muscle tissue.