Development of High Quarity of Chemical Sensors Based on Polymeric Thin Film Coating

聚合物涂层电极（polymer coated electrodes，PCE）是由金属或碳导体直接涂上离子敏感膜、电催化膜或酶膜而成，由于其操作简单、上级电流响应特性、最小化能力等，与传统的膜电极相比，本研究制备了一种新的基于双层膜涂层的电流型葡萄糖和胆固醇传感器，并考察了它们在稳态条件下的传感特性。通过测量H_2O_2或O_2还原的电流，可以监测葡萄糖或胆固醇的浓度。被外部酶膜中的酶反应消耗。获得了葡萄糖或胆固醇浓度与传感器输出之间的可重复性关系。PCE作为电化学检测器， ...更多信息 r用于流动注射分析。由于带正电的电聚合膜与分析物（即阳离子物质（例如，例如，在一个实施例中，多巴胺、1-去甲肾上腺素和DL-3，4-二羟基苯丙氨酸）的反应很小，而中性和阴离子物质（如多巴胺、1-去甲肾上腺素和DL-3，4-二羟基苯丙氨酸）的反应很小。例如，在一个实施例中，3，4-二羟基苯乙酸、4-羟基-3-甲氧基苯乙酸和邻苯二酚）在聚合物涂层电极上的变化与裸电极相比仅轻微。已经在覆盖谐振频率区域的频率范围内进行了各种浓度的水分和支持电解质水介质的测量并以电导谱和磁阻谱的形式表示了结果。例如，电导对频率图和磁阻对频率图）。根据压电石英晶体的电等效电路模型，计算了有关电路参数（电阻R、电感L、电容C和静电电容C_0），特别是支持电解质浓度对电路参数的影响。膜的电解质浓度依赖性溶胀的变化，其特征在于由所评估的参数。实验表明，该方法可以应用于有机、无机和生物薄膜的运动粘度特性和亲和性传感。石英晶体微天平（QCM）在液体中工作时，保持其高的质量灵敏度。因此，QCM已被应用到聚合物薄膜氧化还原过程中发生的质量变化。在用聚（乙烯基二茂铁）、聚苯胺等薄膜修饰的电极的情况下，已经定性地证明了需要离子传输的电中性期间的溶剂传输。

Polymer coated electrodes (PCEs), which consist of a metallic or carbon conductor direct coated with an ion-sensitive, electrocatalytic, or enzyme membrane, are very attractive potentiometric and amperometric sensors due to their simplicity of operation, superior amperometric response characteristics, capability of minimization, etc., compared with conventional membrane electrodes.In this research, new amperometric sensors for determination of glucose and cholesterol based on bilayer film coating have been fabricated and their sensor characteristics under a steady-state condition have been examined. The glucose or cholesterol concentration could be monitored by measuring the current for H_2O_2 or O_2 reduction electrocatalyzed inner film where H_2O_2 was produced orO_2. was consumed by the enzyme reaction in the outer enzyme films. The reproducible relationship between glucose or cholesterol concentration and sensor output was obtained. The PCEs functioned as an electrochemical detecto … More r for flow injection analysis. Substantial unprovement in the selectivity of arnperometric detection for flow injection system was observed as a result of an electrostatic interaction between the positively charged electropolymerized film and analysis, that is, cationic species (e. g., dopamine, 1-norepinephrine and DL-3, 4-dihydroxyphenylalanine) exhibited little response, while the response of neutral and anionic species (e. g., 3, 4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and catechol) was only slightly changed at the polymer coated electrode compared with the bare electrode.The complex admittance measurements of the quartz crystal oscillators with and without a film coating, which are in contact with air, moisture and supporting electrolyte aqueous media of various concentrations have been carried out over a frequency range covering the area of the resonant frequency of each oscillator system, and the results have been represented in the form of conductance and susceptance spectra (i. e., conductance vs. frequency plots and susceptance vs. frequency plots). According to the electrical equivalent circuit model conventionally proposed for a piezoelectric quartz crystal, the related circuit parameters (resistance R, inductance L, capacitance C and static capacitance C_0) have been evaluated, especially as a function of the concentration of supporting electrolyte. The change of electrolyte concentration-dependent swelling of the film was characterized by the evaluated parameters. It is demostrated that the method described can can apply the characterized kinematic viscosity and affinity sensing of the organic, inorganic and biological thin films. The quartz crystal microbalance (QCM) when operated in liquids was shown to retain its high mass sensitivity. The QCM has therefore been applied to the mass changes occurring during redox in thin polymer films. Solvent transport during electroneutrality requiring ion transport has been qualitatively demonstrated in the case of electrodes modified with thin film of poly (vinyferrocene), polyaniline, etc. Less