Elucidation of Mechanisms of HPV Carcinogenesis in Cervical Cancer

人类子宫颈癌的致癌机制尚未阐明。最近，人类乳头瘤病毒(HPV)被认为是一个因素，因为在肿瘤细胞中检测到HPV基因组。我们用分子生物学技术研究了HPV基因组在宫颈肿瘤细胞中的存在。(1)Southern blotting方法在79%的癌前病变(异型增生)中检测到HPV基因组。(2)HPV16DNA通过DNA转导小鼠Balb/3T3细胞。(3)某些类固醇激素促进了转化子的恶性进展。这些结果表明，HPV似乎启动了宫颈癌的发生，一些辅助因素促进了恶变。

The carcinogenesis of human cervical cancer of the uterus is not elucidated. Recently, human papillomavirus (HPV) is considered to be a factor, since the genome of HPV is detected in the tumor cells.We investigated the existence of HPV genome in the tumor cells of the uterine cervix using molecular biological techniques.(1) HPV genome was detected in 79% of the precancerous lesion (dysplasia) by Southern blotting method.(2) A HPV 16 DNA transformed mouse Balb/3T3 cells by transfection of the DNA.(3) Some steroid hormones enhanced the malignant progression of the transformants.These results indicate that HPV seems to initiate the carcinogenesis of cervical cancer and some cofactors promote the malignancy.