A new neurological mutant (creeping) rat : genetic and some neuropathological aspects.

我们发现了一种新的突变大鼠，名为爬行，表现出严重的共济失调。受影响的大鼠在15天内被识别，并在35天内死亡。在出生21天的突变体中，有显着的小脑发育不良，并伴有小脑和大脑中神经元的错位。表型不存在性别差异。遗传学研究表明，爬行性状是由常染色体隐性基因引起的。爬行的大鼠可以在神经体征之前通过弯曲的尾巴来预测。对茜素染色的骨骼的研究显示，尾椎的数量和排列错位大大减少。弯曲尾巴的连锁试验，标志着它非常接近爬行，cre(重组值：0.127；+-gt；0.129)突变型小脑有一些残缺的叶状结构，在神经元定位方面组织结构紊乱。即使在28天时，突变体的小脑层叠也被严重干扰，尽管正常的21天时已经出现了典型的三线形结构。内颗粒神经元数量明显减少，浦基尼细胞位于白质附近。浦基尼细胞也散布在发育不良的内颗粒层中。21d时，突变型小脑GFAP阳性细胞数较正常小脑增多。它们位于颗粒神经元和Purkinie细胞之间。GFAP阳性细胞突起粗大，分支不规则。然而，在28天大的突变小脑中，这些过程变得细长。爬行的大鼠不仅为分析神经元迁移提供了一个有价值的系统，还可能给我们提供一条线索来阐明。GFAP阳性细胞在发育过程中的作用。

We have found a new mutant rats, named creeping, that exhibits severe ataxia. The affected rats are recognized at 15 days and die within 35 days. In the 21-day-old mutant there is a remarkable cerebellar hypoplasia accompanied with malpositioning of neurons both in cerebella and cerebra. There are no sex differences in the phenotype. The genetic study suggest that the creeping traits caused by an autosonal recessive gene.The creeping rats can be predicted by crooked tail prior to the neurological signs. Study of alizarin-stained skeletons showed great reduction in number and misalignments of the tail vertebrae. Linkage tests of crooked tail, symbolized crt, located it extremely close to creeping, cre(recombinant value : 0.127<plus-minus>0.129The mutant cerebelluir had a few rudimentary folia with disorganized tissue architecture in terms of neuronal positioning. The cerebellar lamination was severely disturbed even on 28 days in the mutant, although a typical tri-liniar structure was already present on 21 days in the normal. Inner granular neurons were markedly reduced in number and Purkinie cells were deeply located, i. e., in the vicinity of the white matter. Purkinie cells were scattered among the maldeveloped inner granule layer as well. GFAP-positive cells increased in number in the mutant cerebellum as compared with the normal one at 21 days. They were located among granular neurons and Purkinie cells. Processes of GFAP-positive cells were thick and irregularly branched. However, these processes be came slender in the 28-day-old mutant cerebellum.The creeping rat not only provide a valuable system where the neuronal migration is analyzed but may also give us a clue to elucidate. the functions of the GFAP-positive cells during development.