Dynamic Multi-Spectral Tomography for Kinetic Microspecimen

我们开发了显微测量方法，利用新的光谱和时间切片技术，以观察细胞和毛细管中的化学反应和动力学。具体的研究成果如下：1.四波长激光CT显微镜的研制为了获得样品的光谱信息以及三维图像，我们将四波长激光作为光源引入到我们研制的光学CT显微镜中。验证了光谱信息和三维分布都可以很好地得到.四眼CT显微镜的开发：我们开发了一种四眼CT显微镜，用于观察运动的细胞或生物体。该系统同时采集四个投影，采用非负约束重建算法成功地获得了40 μ m间隔的断层图像.相位CT显微镜的发展：通常动物细胞是透明的，需要染色作为前处理。我们开发了一种相位CT显微镜，用于观察活细胞而无需这种染色过程。三维折射率分布是从几个相衬图像重建。用该显微镜观察培养的烟草细胞。实验结果表明，该系统能够很好地重建断层图像.荧光成像分析显微镜：为了用显微镜观察样品的动态，我们建立了一个分析显微镜，它可以将荧光寿命映射到样品图像上。我们尝试将此系统应用于测量样品的动态响应，从混合物中识别组分，并使用此映射显微镜分离各组分的区域。

We developed microscopic measurement methods utilizing new spectroscopic and time-slicing techniques in order to observe chemical response and dynamics in cell and capillary. The detailed results of this project are as followings :1. Development of Four-Wavelength Laser CT Microscope : In order to obtain spectroscopic information of specimens as well as the 3-D image, a four-wavelength laser was introduced as an optical source into the optical CT microscope we develop. We verified that both spectroscopic information and 3-D distribution could be obtained simultaneously.2. Development of Four-Eye CT Microscope : We developed a four-eye CT microscope for observing moving cells or living organism. In this system four projections were acquired simultaneously, and then 40-mum interval slice images were successfully obtained by a non-negative constrained reconstruction algorithm.3. Development of Phase CT Microscope : Usually animal cells are transparent and staining is required as a prior process. We developed a phase CT microscope for observing alive cells without this staining process. The 3-D refractive-index distribution is reconstructed from several phase contrast images. Cultured tobacco cells were observed with this microscope. It was verified that the slice images were well reconstructed with the system.4. Fluorescence-Mapping Analysis Microscopy : For observing the dynamics of specimens with a microscope, we built an analysis microscope which could map fluorescence lifetime on the sample images. We tried to apply this system to measure dynamic response of specimen, to identify components from a mixture, and to separate area of each components with this mapping microscope.