“Spin-Torch NMR”: Transfer of nuclear hyperpolarization to explore molecular environment

光合作用的电子泵发生在蛋白质称为反应中心（RC）。在这里，（细菌）叶绿素辅因子在高度优化的环境中吸收光和正向电子。光诱导自旋相关自由基对（SCRP）中的自旋演化允许通过固态光CIDNP效应增强NMR信号。瞬态超极化，发生在13 C和15 N原子核的自由基对，将被用来探索分子环境。我们将确定化学位移，化学位移各向异性（CSA），弛豫参数，局部动力学和距离。这些转移计划将进一步发展和应用到两个不同的光合RCs：那些紫色细菌和heliobacteria。比较可能允许识别共同的功能模式。在未来，有了人造RC，这里开发的传输方案也将允许表面研究。

Photosynthetic electron pumping occurs in proteins called reaction centers (RCs). Here, (bacterio)chlorophyll cofactors absorb light and forward electrons in a highly optimized environment. Spin-evolution in the light-induced spin-correlated radical pair (SCRP) allows for NMR signal enhancement via the solid-state photo-CIDNP effect. The transient hyperpolarization, occurring on 13C and 15N nuclei in the radical pair, will be used to explore the molecular environment. We will determine chemical shifts, chemical shift anisotropies (CSAs), relaxation parameters, local dynamics and distances. These transfer schemes will be further developed and applied to two different photosynthetic RCs: Those of purplebacteria and of heliobacteria. Comparison might allow to recognize common functional patterns. In future, having artificial RCs, the transfer schemes developed here will allow for surface studies, too.