Search for xylosylglucose-type sugar chains by pyridylamination

我们发现动物糖蛋白中存在木糖基葡萄糖-0-丝氨酸型糖链，并测定了其完整结构（Hase et al. 1990）。目前尚不清楚这种新型糖链的生物学意义，但这种糖链具有独特的化学结构，并且在蛋白聚糖的连接位置有木糖残基。糖链仅存在于凝血因子中由o糖苷连接的丝氨酸残基上，其中糖链与糖蛋白的egf样结构域相连。为了了解糖链在动物体内的分布，对糖链进行了检测。为此目的，吡啶层析被用于检测极少量的糖链。木糖型糖链主要存在于小鼠和牛的脑、血、肾中，也存在于HepG2细胞株中，尤其是细胞的可溶性部分。在人尿中也发现了三糖。建立了用凝胶电泳法纯化糖蛋白后检测糖蛋白糖链的方法，这是用糖链搜索糖蛋白的基本方法。

We have showed the existence of xylosylglucosyl-0-serine type sugar chain in glycoproteins of animals, and its complete structure was determined (Hase et al. 1990). The biological significance of the new type sugar chains is not known so far, but the sugar chain has unique chemical structure together with the xylose residue in the linkage position of proteoglycans. The sugar chains are found only on the serine residue linked by O-glycosidically in blood coagulation factors, where the sugar chains are linked to the EGF-like domain of the glycoproteins. To know the distribution of the sugar chains in animals, detection of the sugar chains was carried out. For this purpose pyridylamination was used to detect very small amounts of sugar chains. Xylosylglucose type sugar chains were found mainly in brain, blood, and kidney of mouse and ox. And the sugar chains were also found in cell line HepG2, especially in the soluble fraction of the cells. In human urine the trisaccharide was also found. The procedure was developed by which sugar chains of the glycoproteins are detected after purification of the glycoproteins by gel-electro-phoresis, which is a base procedure to search glycoproteins with the sugar chains.