Structure, Localization and Function of Proteoglycan Expressed Specifically in the Developing Rat Brain

(1)我们提出了一种识别Neurcan的单抗MAb-1G2，它是一种脑特异性的硫酸软骨素蛋白多糖，在大鼠脑中具有220 kDa的核心糖蛋白。(2)抗体(Pab-291)在兔体内培养成一种合成肽，其氨基酸序列与Neurcan的一部分(氨基酸编号483至498)相同。(3)利用这些抗体，发现Nucan主要存在于未成熟的脑中。免疫细胞化学和原位杂交实验均显示，在躯体感觉区桶形成期，神经钙素的合成在转录水平上减少。(5)用纯化的神经钙素和这些抗体进行的细胞生物学研究表明，神经钙素参与了神经突起的分支和伸长的调节。

(1) We raised a monoclonal antibody, named MAb-1G2, which recognized neurocan, a brain-specific chondroitin sulfate proteoglycan with a 220 kDa core glycoprotein, and its C-terminal half in the rat brain.(2) Antibodies (PAb-291) were raised in the rabbit to a synthetic peptide whose amino acid sequence is identical to a portion (amino acid No. 483 to 498) of N-terminal half of neurocan.(3) Using these antibodies, nuerocan was shown to exist predominantly in inmature brain, but to exist as two proteoglycan fragments in mature brain.(4) Both immunocytochemical and in situ hybridization experiments revealed that the synthesis of neurocan was reduced at transcriptional level during barrel formation in the somatosensory area.(5) Cell biological studies using purified nerocan and these antibodies suggested that neurocan was involved in regulation of branching and elongation of neurites.