Study on virus etiology of dilated cardiomyopathy

应用聚合酶链反应（PCR）和原位杂交（ISH）技术检测了科萨基B3病毒心肌炎动物模型和97例心肌炎（MC）、扩张型心肌病（DCM）或其他心脏疾病患者心肌活检组织中肠道病毒RNA的存在。在C3 H/He小鼠中，直到接种的第28天才检测到病毒基因组，而在A/J小鼠中，直到第56天才检测到病毒基因组。急性MC和A/J小鼠在第56天留下炎性细胞浸润后，两种品系的小鼠都发生了严重的心肌纤维化。在接种病毒的仓鼠中，检测到的病毒基因组长达180天，有些仓鼠已经发展出DCM样特征。ISH在心肌细胞和心肌病变内及周围的间质细胞中鉴定出病毒基因组。在人类活组织检查中，21%的患者检测到肠道病毒基因。在MC、DCM和其他心脏疾病中，基因组的检出率分别为23%、18%和22%。但基因组阳性的活检并不总是显示MC的组织学表现。一些结果可能支持肠道病毒感染和扩张型心肌病之间的联系。

We examined myocardial tissues for the presence of enteroviral RNA in animal models with coxsackie B3 virus myocarditis and myocardial biopsies from 97 patients with myocarditis(MC), dilated cardiomyopathy(DCM) or other cardiac diseases using polymerase chain reaction(PCR) and in situ hybridization(ISH). In C3H/He mice the virus genome was detected up to the 28th day of inoculation, whereas in A/J mice up to the 56th day. Both strains of mice developed severe myocardial fibrosis after the acute MC and A/J mice had left inflammatory cell infiltrations at the 56th day. In hamsters inoculated with the virus, the viral genome detected up to the 180th day and some had developed DCM-like features. The virus genome was identified by ISH in myocardial cells and some interstitial cells in and around the myocarditic lesions. In human biopsies enteroviral genonme was detectedin 21% of all patients. In MC, DCM and other cardiac diseases the genome was detected in 23%, 18% and 22%, respectively. But the genome-positive biopsies did not always showing histologic findings of MC. Some results may support a link between enteroviral infection and DCM.