Experimental radiological study for prevention and control of metastasis

本研究的具体目标是建立C3 H/HeJ小鼠肿瘤淋巴结转移系统，并研究辐射对淋巴结转移的影响。将命名为NFSA 2ALM 1纤维肉瘤和NR-S1鳞状细胞癌的自发起源的小鼠肿瘤系统接种在后腿的足垫中。转移性淋巴结肿大（通过组织学证实）在肿瘤细胞接种后约2周检测到，而克隆性肿瘤细胞在接种后10天左右开始释放到血液循环中，3-4周后出现肉眼可见的肺结节。这些结果支持并扩展了先前研究的结论：1）局部肿瘤照射对克隆源性肿瘤细胞释放（CTCR）进入血液循环和对肺转移发展的抑制作用，2）CTCR效率可预测转移效率，即高CTCR与肺或淋巴结自发转移效率相关。

The specific goal of the present study was to establish lymphnode metastasis system(s) using C3H/HeJ mouse tumors and to investigate irradiation effect on metastatic development to lymphnodes. Mouse tumor systems of spontaneous origin named NFSA2ALM1 fibrosarcoma and NR-S1 squamous cell carcinoma were inoculated in the footpad of a hind leg. Metastatic lymphnode enlargement (confirmed by histology) was detected by about 2 weeks after the tumor cell inoculation, while clonogenic tumor cell release into the blood circulation started around 10 days and macroscopic lung nodules developed after 3-4 weeks of the tumor cell inoculation.Local tumor irradiatioon was found to suppress lymphnode metastatic development dose-dependently between 10 and 40 Gy.These results support and extend the conclusion of the previous study regarding 1) suppressive effect of local tumor irradiaiton on the clonogenic tumor cell release (CTCR) into the blood circulation and on the metastatic development to the lung, and 2) CTCR effeciency may predict the metastatic efficiency, i.e. high CTCR correlates to high spontaneous metastasis efficiency to the lung or lymphnodes.