A study of the alpha-subunits of brain GTP-binding proteins from patients suffering schizophrenic, or Alzheimer's disease

采用高灵敏的酶免疫分析法，研究了gtp结合蛋白α亚基、氧化石墨烯（Go α）和Gi2 （Gi2alpha）在对照组和精神分裂症死后大脑6个区域的浓度。与对照组相比，精神分裂症患者右半球海马体中的α显著降低；SAS分析显示了显著的诊断x侧相互作用。在大脑的其他区域，根据诊断、侧面、年龄、性别和死后延迟进行分组分析显示，对照组和精神分裂症患者之间的Goalpha没有显著差异。Gi2alpha的浓度在各区域无显著差异。这些发现与adp核糖基化的结果形成对比，后者显示对侧（左）半球海马和壳核中百日咳毒素adp核糖基化的量减少。这种差异可能是由于精神分裂症患者左右侧海马体中存在不同的Go或Gi1构象。

Concentrations of the alpha subunits of GTP-binding protein, Go (Goalpha) and of Gi2 (Gi2alpha) in 6 areas of control and schizophrenic post-mortem brains were investigated using the highly sensitive enzyme immunoassay method. There was a significant decrease in Goalpha in the hippocampus of the right hemisphere in schizophrenic patients compared to controls; SAS analysis demonstrated a significant diagnosis x side interaction. In other areas of the brain, analysis by grouping under diagnosis, side, age, gender and postmortem delay showed no significant deviations in Goalpha between controls and schizophrenics. The concentrations of Gi2alpha did not differ significantly in any area. These findings contrasted with the results yielded by ADP-ribosylation, which showed decreased pertussis toxin ADP-ribosylated amounts in the hippocampus and putamen of the contralateral (left) hemisphere. The discrepancy might be explained by the existence of differing conformations of Go or Gi1 in the hippocampi of the right and left sides in schizophrenics.