The study of using cultured fibroblast cells to the preiodontal tissue regeneration.

对于牙周破坏缺损的再生，有必要保留一定数量以上的细胞。特别是来源于牙周韧带的成纤维细胞样细胞对牙槽骨、牙骨质和牙周韧带的再生具有重要作用。本研究取犬外拔牙表面的牙周韧带细胞。体外连续培养4-6次后，用荧光标记试剂盒（Zynaxis， USA）对成纤维细胞样细胞进行染色。成纤维细胞样细胞染色良好。染色的细胞在进一步培养后，被认为这些细胞分裂后的细胞核几乎与原细胞核染色相同。这些细胞被放置在同一只狗的分叉区缺损处。从前庭侧进行皮瓣手术以保持上皮附着。自体胶原凝胶移植后的骨量高于对照组。成纤维细胞样细胞掺入胶原凝胶比单独掺入胶原凝胶要高。

It's necessary to keep more over absolute number of cells for the regeneration of periodontally destructed defects.Especialy, fibroblast like cells sourced from periodontal ligaments have important part for the regeneration of alveolar bone, cementum and periodontal ligament.In this study, it had taked the periodontal ligament cells from extraeted teeth surface of the dog.The fibloblast like cells were dyed by fluorescent marker kit (Zynaxis, USA) after 4-6 successive cultured in vitro. The fibroblast like cells were well dyed.The cells that dyed had been cultured futher ore, it was assumed that devided nucleus of these cells were almost the same dyed as originals.These cells were in placed the defects of the furcation area of the same dog.Flap procedure were made from vestiblular side to keep epithelial attachment.The bone of implanted collagen gel by itself was higher than control.Fibroblast like cells mixed into collagen gel was higher than collagen gel by itself.