Study on glutelin genes of storage proteins in rice.

水稻谷蛋白的两个主要亚基，酸性（α）亚基和碱性（β）亚基，其表观分子量和pI值各不相同，SDS-PAGE和IEF分析发现，不同类型水稻谷蛋白的表观分子量和pI值存在较大差异，其中粳稻和籼稻谷蛋白的表观分子量和pI值差异较大。对粳稻品种金迷宫和籼稻品种IR 36的谷蛋白进行了比较准确的分析。SDS PAGE分析表明，谷蛋白分为α 1、α 2、α 3和α 4四条亚基带，β 1、β 2、β 3和β 4四条亚基带。在IEF分析中，α和β亚基分别至少有12和9条带。在两个亚基中都检测到了几个多态性手。通过双向电泳分析多肽的表观分子量与等电点的关系。每个SDS-PAGE条带由显示pI异质性的一些多肽组成，而一些多肽 ...更多信息 相同pI的分子量不同。IEF中的多态性条带被检测为每个单点，并且属于α-2和β-2。利用IEF技术对“Kinmaze”、“IR 24”和RI（重组自交系）的多态性条带进行遗传分析。“IR 24”与“IR 36”的电泳图谱基本相同，但在α亚基上有另一条带。RI系由“Asominori”（显示与“Kinmaze”相同的模式）和“IR 24”之间的杂交种子制成。“金迷宫”与“IR 24”正反交F1种子的带型表现出基因剂量效应。F_2分析中，谱带的分离与表型的染色强度符合1：1：1：1（或3：1）的比例，表明这些谱带受不完全显性基因控制。条带间存在互补带，表明每条带受各不完全基因控制。在控制α 2多肽和α 3、β 2多肽的基因中，发现了控制每个多态性条带的基因之间的连锁关系。然后利用RI系对这些基因进行了染色体定位。控制α-2多肽的基因显示与位于1号染色体上的RFLP标记连锁，而控制α-3和β-2多肽的基因显示与位于2号染色体上的RFLP标记连锁。少

Two major subunits of rice glutelin, the acidic (alpha) and basic (beta) subunits consist of various polypeptides in their apparent molecular mass and pI.We found a wide variation among the rice atrains in the apparent molecular mass and pI by SDSPAGE and IEF,especially between Japonica and Indica types. The glutelin of "Kinmaze", Japonica type, and "IR36", Indica type, were analyzed exactly. In SDSPAGE analysis, the glutelin was separated into four alpha subnit bands, alpha-1, alpha-2, alpha-3 and alpha-4, and four beta subunit bands, beta-1, beta-2, beta-3 and beta-4.The alpha-3 of "Kinmaze" moved faster than that of "IR36". In IEF analysis, alpha and beta subunits had at least 12 and 9 bands, respectively. Several polymorphic hands were detected in both subunits. The relation between the apparent molecular mass and pI of the polypeptides were analyzed by 2-dimensional electrophoresis. Each SDS-PAGE band consists of some polypeptides showing pI heterogeneity, while some polypeptides … More of same pI showed different molecular weight. The polymorphic bands in IEF were detected as each single spot, and belong to alpha-2 and beta-2. The genetic analysis of the polymorphic bands were done by IEF using "Kinmaze", "IR24" and RI (recombinant inbred) lines. "IR24" shows almost the same pattern with "IR36", but has another band in alpha subunit. RI lines were made from a cross seed between "Asominori", showing the same pattern with "Kinmaze", and "IR24". The bands of reciprocal F1 hybrid seeds between "Kinmaze"and "IR24"showed the gene dosage effect. In F2 analysis, segregation of the bands, phenotypes in staining intensity, fitted to a ratio of 1 : 1 : 1 : 1 (or 3 : 1), indicating these bands were controlled by incomplete dominant genes. Recombinants were found among the bands, indicating each band was controlled by each incomplete gene. The linkage relationship of the genes controlling each polymorphic bands were found in both genes controlling alpha-2 polypeptides and alpha-3 and beta-2 polypeptides. Then chromosomal location of these genes was studied by means of RI lines. The genes controlling alpha-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 1, while The genes controlling alpha-3 and beta-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 2. Less