Axnic cultures of rust fungi from the haploid state

1.冠锈菌的轴向培养。Coronata和P.recondita f.tritici是在含有25%浓度的Murashige&Skoog矿物质和维生素、蔗糖、蛋白胨、Lab-Lemco肉汤和琼脂的人工培养基上建立的。显微镜观察和菌丝细胞细胞核DNA含量测定表明，培养物为单倍体、单核细胞。冠冠P.coronata var.15对单担子培养物在所有可能的组合中，Coronata被放置在介质上，间隔约1 mm。对峙1个月后观察接触区菌丝细胞核数时，观察到单核菌丝自交组合。另一方面，在不同培养物间杂交组合中，90.5%可观察到双核菌丝。如果接触区存在双核菌丝，则认为两个分离物是亲和的；如果没有双核菌丝，则认为两个分离物是不相容的。该真菌的交配型可由A、B两个不亲和性因子控制的多异态四极不亲和性来判断。将单个担孢子侵染总状柏化学菌叶片产生的精原置于冠冠白僵菌的15个单担孢子培养物上。科罗纳塔。在45次交配试验中，有7次在叶片上产生了AECIA。在AECIA中产生的孢子能够侵染高山龙须草。

1. Axnic cultures of Puccinia coronata var. coronata and P.recondita f.sp.tritici were established by placing aseptic leaf segments with spermogonia originated from a single basidiospore infection on the artificial medium containing a 25 % concentration of Murashige & Skoog's minerals and vitamins, sucrose, Peptic peptone, Lab-lemco broth, and agar.2. Microscopic observation and measurement of DNA content of the nucleus in hyphal cells of the cultures showed that they were haploid and uninucleate.3. Pairs of 15 single-basidiospore cultures of P.coronata var. coronata were placed approximately 1 mm apart on the medium in all possible combinations. When the nuclear number of hyphal cells in a contact zone was investigated one month after confrontation, monokaryotic hyphae were observed in selfing combination. On the other hand, dikaryotic hyphae were observed in 90.5% of crossing combinations between different cultures. Two isolates are considered compatible if dikaryotic hyphae are present in the contact zone but are incompatible if dikaryotic hyphae are absent. The mating type of the fungus could be estimated by multiple-allelomorphic tetrapolar incompatibility controlled by the "A" and "B" incompatible factors.4. Spermogonia originated from a single basidiospore infection on a leaf of Berchemia racemosa were placed on 15 single-basidiospore cultures of P.coronata var. coronata. Aecia were produced on the leaf in 7 mating tests out of 45. Aeciospores produced in the aecia were capable of infectiong Calamagrostis arundinacea.