A selective labelling of the olfactory neuron by retrovirus with the foreigen genes and contral projection of the labelled olfactory neuron.

将pZip-Neo-CAT(氯苯乙酰转移酶)载体转化为缺乏包装信号的pSI^-细胞系。用新霉素类似物G418对含CAT基因的psi~-细胞株进行分离，检测其CAT活性。建立了一株产病毒细胞系(8-7)。通过培养产生病毒的细胞，获得含有外源基因的病毒。使用成年大鼠。在麻醉下，摘除嗅球。在嗅球切除术(OBX)后的四天内，将猫引入的病毒导入动物体内。病毒感染1周后，用4%多聚甲醛固定动物。冰冻切片切开。切片用抗CAT抗体孵育。在OBX大鼠中未发现CAT阳性的嗅觉神经元。产生病毒的细胞系的滴度可能不够高，或者病毒包膜基因的感染力太弱。

A pZip-Neo-CAT(chlormaphenical acetyl transferase) vector was transformed into a psi^- cell line which lacked a packaging signal. The psi^- cell line with the CAT gene was segregated by G418 (neamycin analogue) and its CAT activity was checked. A virus producing cell line (8-7) was established. Viruses with the foreigen genes were harvested by culturing the virus producig cells.The adult rats were used. Under anesthesia the olfactory bulb was removed. In four days after olfactory bulbectomy (OBx), animals were transfected with the CAT-introduced viruses. One week after viral transfection, animals were fixed with 4% paraformaldehyde. Frozen flontal sections were cut. The sections were incubated with an antibody against CAT.CAT-positive olfactory sensory neurons were not found in OBx rats. Titers of the virus producing cell line might not be high enough or infection force of virus env gene would be too weak.