In vitro regeneration ability and functioning differantiation of ciliated epithelial cells in odontogenic maxillary lesions

用外植体培养法培养胃窦粘膜碎片。圆形细胞的每一个细胞质都被认为是基细胞，并带有细胞极性。从肌动蛋白的分布和定位来看，在胞质伸展的周边处可观察到纤毛的形成，导致纤毛向纤毛柱状细胞生长发育。使用与上皮角化有关的细胞标记物，基底细胞在体外显示出向成熟的纤毛细胞和/或鳞状细胞或鳞状化生分化和生长的潜力。实验证据表明，在组织学诊断中，胃窦癌以鳞状细胞癌的形式发生。在本研究中，通过高速视频拍摄的纤毛细胞培养现象，清楚地分析了纤毛运动的机制。此外，本研究还解释了纤毛异常形成的发生机制。自1993年以来，上述成果部分以口头报告的形式发表，现在准备报告一些论文，如不同地点所示。

Fragments of antral mucosae were caltivated by explant culture method. Each Cytoplasm of round cells thought to be basal cells extended with cellular polarity. At the peripheral site of extending cytoplasm, cilia formations were observed resulting in growth and development in shape and function towards ciliated columnal cells from the states of actin distribution and its localization. Basal cells showed in vitro direct evidence of potentialities to differentiate and grow towards mature ciliated cells and/or squamous cells, or squamous metaplasia, using cell markers concerning epithelial keratinization. The experimental evidence suggests antral cancers occur as squamous cell carcinoma in histologic diagnosis. In this study, mechanism of ciliary motion was clearly analyzed from phenomena of cultured ciliated cells taken by high speed video. Furthermore, an occurrence mechanism of abnomal cilia formation was explained in this study. Since 1993, above results were partly published by oral presentations and are now prepared to report some papers, as shown in different place.