Basic study of heart rate variability using chick embryo

为了弄清鸡胚心率变异性(FHRV)与环境条件的关系，用鸡胚进行了如下模拟实验：1)非侵入性地从鸡胚中提取心跳；2)生成鸡胚心率图；3)控制实验箱中的温度和湿度；4)改变温度测量鸡胚HRV；5)O_2和CO_2对鸡胚HRV的依赖性；6)胚胎运动对鸡胚HRV的依赖性；7)其他环境因素(强磁场、电磁波、超声波)对鸡胚心率变异性的影响。孵化4d后，用光学体积描记仪和心音图两种方法检测心率变异性，得到心率变异性与温度、O2、CO2浓度和胚胎运动等环境因素变化的关系。用鸡胚胎进行的模拟实验结果与人类胎儿心率变异性(FHRV)相似。

To make clear the relation of fetal heart rate variability (FHRV) with the environmental conditions, simulation experiments using chick embryo were made in following subjects.1) picking up heart beat noninvasively from chick embryo2) producing the cardiotacogram of chick embryo3) control temperature and humidity in experimental box4) measurement of HRV of chick embryo while changing temperature5) dependency of O_2 and CO_2 on HRV of chick embryo6) dependency of embryo movement on HRV of chick embryo7)influence of other environmental factors (intensive magnetic field, electro-magnetic wave, ultrasonic wave) to HRV of chick embryoThe subjects of 1), 2) and 3) which is bearing basic instrumentation were realized. Heart beat was detected by the two methods of optical plethysmograph and phonocardiograph from 4 days after incubation, and the heart rate variability was obtained in relation to change of the environmental factors such as temperature, O_2 and CO_2 concentration and embryonic movement. The results of the simulation experiments by chick embryo showed the analogous phenomena to fetal heart rate variability (FHRV) of human being.