Study on neutrophil functions in infectious diseases in a allergic diseases and analysis of intracelluar signal transmission

慢性肉芽肿性疾病(CGD)是一种典型的遗传性吞噬细胞疾病，迄今已从吞噬细胞的角度对其进行了广泛的评估，但从淋巴细胞功能的角度进行了充分的研究。我们的研究已经证实了CGD患者淋巴细胞亚群的差异，这有望对阐明疾病的病理生理有所帮助。(2)甲氧苄啶-磺胺甲恶唑治疗成功地治疗了1例高免疫球蛋白E综合征。高免疫球蛋白E综合征的病因仍未阐明，是一种独特的综合征，尤其是在感染和过敏方面。我们经历的患者有反复的细菌感染发作，这些感染对传统的抗菌药物具有耐药性。磺胺甲恶唑联合甲氧苄啶(ST)治疗大多数CGDp-…已有临床疗效更多的病人，已经成功地应用于这位病人。临床改善也与一些中性粒细胞功能正常化以及显著升高的血清IgE水平下降有关。胎粪诱导肺损伤主要是通过激活肺泡巨噬细胞介导的。为了阐明胎粪诱导细胞损伤的机制，我们观察了胎粪对兔肺泡巨噬细胞(AM)的影响。AM与胎粪共培养时，AM超氧阴离子生成量明显增加。结合一系列其他实验的结果，我们的结果提示胎粪诱导的肺损伤可能是通过激活肺泡巨噬细胞而发生的，巨噬细胞-上皮细胞轴可能在胎粪诱导损伤的发病机制中起重要作用。(4)兔肺泡巨噬细胞产生超氧阴离子的成熟变化。了解与年龄相关的细胞功能的变化对于解释新生儿的一些生物学现象是重要的。利用兔肺泡巨噬细胞，我们发现，即使在幼兔体内使用一种直接刺激蛋白激酶C的趋化肽，其产生超氧化物的能力也得到了充分的发展。

(1) Decreased CD4+CD29+ (memory T) cells in patients with chronic granulomatous disease.Chronic granulomatous disease (CGD) , a representative hereditary phagocyte disorder, has so far been extensively evaluated from the aspect of phagocytes but has not been duly studied in terms of lymphocyte functions. Our study has demonstrated differences in lymphocyte subsets in patients with CGD,which may hopefully be of some contribution in clarifying pathophysiology of the disease.(2) Successful trimethoprim-sulfamethoxazole therapy in a patient with hyperimmunoglobulin E syndrome.Hyperimmunoglobulin E syndrome, of which etiology still remaines to be elucidated, is one of the unique syndromes especially in terms of infections as well as allergy. The patient we experienced had repeated episodes of bacterial infections which were resistant to conventional antimicrobial medications. Sulfamethoxazole-trimethoprim (ST) therapy, of which clinical efficacy has been established in the majority of CGD p … More atients, has been successfully employed in this patient. Clinical improvement was also associated with normalization in some neutrophil functions as well as a decrease in markedly elevated serum IgE level. Although it was small and anecdotal, this study can certainly be of help in understanding the nature of this enigmatic syndrome.(3) Meconium-induced lung injury mediated by activation of alveolar macrophages.To clarify the machanism of meconium-induced cellular injury, we examined the effect of meconium on the rabbit alveolar macrophages (AM) . Superoxide anion production of AM significantly increased when AM were cultured with meconium. When combined with those of a series of other experiments, our results suggest that meconium-induced lung injury may occur through an activation of alveolar macrophages and that the macrophage-epithelial cell axis may be important for the pathogenesis of meconium-induced injuries.(4) Maturational changes in superoxide anion production of rabbit alveolar macrophages.Understanding of changes of age-related celluar functions are important in interpreting a number of biological phenomena in newborns. Using rabbit alveolar macrophages, we showed that the ability of superoxide production is sufficiently developed even in young rabbits with a chemotactic peptide which directly stimulates protein kinase C. Less