Combination Therapy of Hyperthermia and Immunotherapy with Radiation to Cancer.

对于晚期癌症，我们研究了一种新的方案，即免疫治疗（链球菌制剂，OK-432）和热疗联合放疗。两种疗法都是从热丹毒后恶性肿瘤有时消退消失的经验出发的。已知热致敏辐射效应并产生热休克蛋白（HSP）诱导热耐受性。有报道称，注射OK-432可诱导热休克蛋白产生并激活γ - δ - t细胞对抗热休克蛋白。A)动物实验：实验肿瘤为兔Vx-2癌。辐射是10兆电子伏特x射线。在肿瘤周围和肿瘤内注射OK-432。实验组为a)单独放疗，b)放疗+热疗，c)OK-432+放疗，d)OK-432+放疗+热疗。a组和b组肿瘤暂时消退，但再次生长，所有兔子死亡。c、d组25周肿瘤均明显消退、消失。随着辐射剂量的降低，c组的抗肿瘤活性下降。B)临床研究：1)皮内注射OK-432后，γ - δ - t细胞略有增加。2)经瘤内注射，26%的患者外周血γ - t细胞显著升高，26%的患者外周血γ - t细胞在所有患者中显著升高。3)经c组治疗，1例横纹肌肉瘤消失后复发。病例2手术后胃癌锁骨上转移大而不能手术，病例3男性手术后乳腺癌大面积皮肤转移经d组治疗均完全消退。免疫疗法和热疗联合放疗在动物和临床研究中均显示出相同的结果。这些结果表明，这种联合疗法对晚期癌症非常有用。

For advanced cancer, we studied a new protocol that both immunotherapy (Streptococus preparation, OK-432) and hyperthermia were combined with radiation. Both therapy began from experiences that malignant tumor sometimes regressed and disappeared after Erysipelas with heat. It was known that heating sensitized radiation effects and produced Heat Shock Protein (HSP) induced thermotolerance. And it was reported that OK-432 injection induced HSP production and activated gammadelta-T cell against HSP.A)Animal studies : Experimental tumor was rabbit's Vx-2 carcinoma. Radiation was 10 MeV X-ray. OK-432 was injected arround and in tumor. Experimental group was a)Radiation alone, b)Radiation+Hyperthermia, c)OK-432+Radiation and d)OK-432+Radiation+Hyperthermia.In group a and b, tumors temporally regressed but regrew again and all rabbits died. In group c and d, all tumor remarkablly regressed and disappeared for 25 weeks. By a reduction of radiation dose, the antitumor activity of group c was declined.B)Clinical studies : 1)By intradermal injection of OK-432, gammadelta-T cell slightly increased. 2)By intratumoral injection, gammadelta-T cell in 26% of patients remarkablly increased in peripheral blood, and gammadelta-T cell in 26% of patients remarkablly increased in all patients. 3)Recurrenced rhabdo-myosarcoma in case one was disappeared by group c pattern's therapy.In case 2, big and inoperable supraclavicular metastasis of operated stomach cancer and in case 3, wide spreaded skin metastasis of male operated breast cancer were completely regressed by group d pattern's therapy.Combination therapy of immunotherapy and hyperthermia with radiation showed the same results on big rumors in animal and clinical studies. These results showed that this combination therapy would be importantly useful for advanced cancer.