The action mechamisms of bioactive substances in follicular fluid related to human ovarian folliculogenesis and dysfunction

卵巢和卵泡发育的功能受激素和局部自分泌/旁分泌因子的调节。卵泡液中含有多种生物活性物质。本实验室从猪和/或牛卵泡液中分离出抑制素、激活素和卵泡抑素。成功克隆了人FSH受体、人LH/CG受体和大鼠激活素受体II。研究卵巢中产生的内分泌、旁分泌和自分泌因子对阐明卵泡发生的调控机制具有重要意义。本研究尝试从卵泡液中分离纯化生物活性物质，并对生物活性物质和促性腺激素受体的功能进行了研究。虽然抑制素在人卵泡液中的含量很低，但用免疫亲和层析法从人卵泡液中纯化得到了纯度较高的抑制素。激活素对促性腺激素受体的作用研究表明，激活素A可促进FSH受体mRNA的表达，促进FSH诱导的LH/CG受体mRNA的表达，激活素是促卵泡生成素的最强激活剂，cAMP和PMS均能促进促卵泡生成素的生成。

The function of ovary and folliculogenesis is regulated by both hormones and local autocrine/paracrine factors. Many kinds of bioactive substances exist in follicular fluid. Inhibin, activin and follistatin from porcine and/or bovine follicular fluid were isolated in our laboratory. We are successful in cloning of human FSH receptor, human LH/CG receptor and rat activin receptor type II,also. In order to elucidate the regulatory mechanisms of folliculogenesis, it is important to study the endocrine, paracrine and autocrine factors produced in ovary. We tried to purify bioactive substances from follicular fluid and studied functions of bioactive substances and gonadotropin receptors. Inhibin was purified to homogeniety from human follicular fluid with an immunoaffinity chromatography though it's concentration was much lower in human follicular fluid. The study of activin on gonodotropin receptors demonstrated that activin-A enhanced the FSH receptor mRNA and the FSH induced LH/CG receptor mRNA.We also demonstrated that actvin was the strongest activator of follistatin production and that both cAMP and PMS facilitated follistatin production.