An environmental medical study on the induction of metallothionein under stressful conditions

本研究的目的是首先确定金属硫蛋白的合成是否受到各种环境应激因素（如噪音、振动、固定或饥饿）的诱导。该研究的第二目标是通过检查金属硫蛋白的性质、蛋白质同种异构体的组成、蛋白质在体内的分布和组织中的变化来阐明蛋白质在应激条件下的作用。采用生物色谱和离子交换色谱相结合的方法，建立了异金属硫肽的鉴定和定量方法。我们证明，暴露于噪音、振动、固定和饥饿的大鼠肝脏中，金属硫蛋白的合成被诱导，蛋白质含量增加。

The goal of this study was firstly to determine whether the synthesis of metallothioneins was induced by various environmental stressors such as noise, vibration, immobilization, or starvation. The goal of the study was secondly to elucidate the role of the proteins on stressful conditions by examining natures of metallothioneins, composition of the isoforms of the proteins, distribution of the proteins in the body, and variations in tissues. The method for the identification and quantification of the isometallothioneins was established by the combination of the bio-chromatography system and ion-exchange chromatography. We demonstrated that the synthesis of metallothioneins was induced and the content of the protein increased in the livers of rats exposed to noise, vibration, immobilization, and starvation.