Construction of Monomolecular Membrane with Different Surface Properties and Electron Transfer

将内外表面具有不同表面性质的单分子膜制备成囊泡。本研究的目的是研究这种分子组装体的特性，并构建功能性囊泡包裹浓缩蛋白质，如血红蛋白。将两端具有不同头部基团的两亲性分子通过超声分散到水中，以制备具有单分子层的约100 nm的囊泡。通过TEM观察、囊泡的总体积测量与计算体积的比较，以及通过将Eu^<3+>加入到外部水相中而使^1H-NMR中胆碱甲基质子峰发生位移，证实了这一点。所得囊泡非常稳定，并且没有观察到囊泡大小的变化和水性内容物的渗漏。如果尺寸减小或浓缩的血红蛋白被封装，则膜中的两亲物的取向被调节。通过在磷脂膜表面加入还原剂，使膜内氧化剂被还原，从而实现了膜内电子传递，并将两端带有离子基团的石胆酸衍生物引入磷脂膜。一个显着的影响，它的分子包装和囊泡悬浮液的稳定性增强澄清的荧光去极化方法，1H-NMR，和浊度测量。

Monomolecular membrane having different surface properties between inner and outer surface was prepared as vesicles. The purpose of this research is to study the characteristics of this molecular assemblies and to construct functional vesicles encapsulating concentrated protein such as hemoglobin. Amphiphilic molecules of which both ends are different head groups were dispersed into water by sonication to prepare vesicles of ca.100 nm with monomolecular layr. This was confirmed from TEM observation, the measurement of the total volume of the vesicles in comparison with calculated volume, and the shift of choline methyl proton peak in ^1H-NMR by the addition of Eu^<3+> to outer aqueous phase. The resulting vesicles were very stable, and no change of vesicular size and no leakage of aqueous contents were observed. The orientation of the amphiphiles in the membrane was regulated if the size was reduced or if concentrated hemoglobin was encapsulated. Electron transfer through the membrane was analyzed from the reduction of inner oxidants by adding reductant to the outer aqueous phase.A lithocholic acid derivatives with ionic groups at both ends was incorporated to the phospholipid membrane. A significant influence of it on the molecular packing and the stability enhancement of the vesicle suspension were clarified by a fluorescence depolarization method, 1H-NMR,and a turbidity measurement.