Electrophoretic studies on Bruguiera gymnorrhiza and Kndelia candel in Okinawa, Japan

本研究尝试建立垂直平板聚丙烯酰胺凝胶电泳（PAGE）技术，用于红树林树种同工酶位点分析。研究中使用了两种红树林树种：木榄和秋茄。两种均广泛分布于日本的冲绳，尤其在亚洲热带国家也有分布。PAGE分析的样品也取自西表岛、石垣岛、宫古岛和冲绳天然红树林种群的成熟叶片。改进了聚丙烯酰胺凝胶染色技术，使酶谱清晰，分离胶占7.5%，间隔胶占3.5%。对酸性磷酸酶（ACP）、酯酶（EST）、谷草转氨酶（GOT）、葡萄糖磷酸变位酶（PGM）、过氧化物酶（POD）等17种酶进行了染色，得到了7种酶的酶谱。表型显示，在不同的个体中，电变体作为等位酶。对ACP、EST、PGM、GOT等基因座进行了检测。这些基因均为非单体或二聚体，但其中部分基因表现为无效表型，尚不清楚其基因频率。因此，获得的基因频率比预期的要少。但是遗传距离正在被尝试计算。

In this study, and attempt has been made to develop techiniques of the polyacrylamide gel electrophoresis (PAGE) of a vertical slab-format to analyzing isozyme loci of mangrove tree species. Two mangrove tree species were used in the study : Bruguiera gymnorrhiza and Kndelia candel in the family Rhizophoraceae. Both of two species widely are distributed in Okinawa, Japan, and distributed especially in the tropical Asian countries too. The samples of PAGE were used matured leaves of each tree collected from natural mangrove populations on Iriomote, Ishigaki, Miyako islands and Okinawa island too. Staining techniques were improved to get zymogrames clearly on the polyacrilamide gels which were consisted with 7.5% of the separation gel and 3.5% of the spacer gel. Seventeen enzyme species were stained such as acid phosphate ((ACP), eaterase (EST), gulutamate oxaloacetatate transamirase (GOT), phosphoglucomutase (PGM), peroxidase (POD), etc. Fine zymogrames were obtained 7 enzyme species out of 17 enzyme species. Phenotypes showed elecromorphs as allozymes in different individuals. Several loci of ACP,EST,PGM,GOT were estimated. All of them were either nonomers or dimers, but some of them showed null phenotypes which were not gotten exact gene frequencies. For this reason, the obtained gene frequencies were less numbers which were expected. But genetic distances are being tried to calculate.