LC-MS-QTOF-System

特里尔大学第六学院“区域与环境科学”的几个系申请了一套高分辨率液相色谱-质谱（LC-MS-QTOF）系统。该系统将(i)取代旧的液相色谱-串联质谱系统（自2006年开始使用），（ii）为更具选择性和敏感性的分析提供新的选择，特别是能够筛选分析人为和自然来源的物质的复杂混合物。正在进行的和计划好的项目证明了对这种分析的需求是合理的。作为特里尔大学唯一的这种分析系统，它为分析设备提供了强大的丰富，并将分别在部门和实验室集群中合作使用，就像现有设备的情况一样。使用质谱法进行环境分析多年来一直在土壤科学系（申请人）以及共同申请部门和学院其他部门进行研究和教学。上述液相色谱-串联质谱系统在购买之日提供了非常好的分析选择，大大促进了各种资助项目的获得。由于其使用寿命，系统不再可靠；停机时间和维修时间增加，与常规操作和有限时间项目的使用相冲突。因此，我们申请了一种液相色谱-质谱联用系统，该系统能够高分辨率地测定未知化合物，也可用于常规检测和定量已知化合物（“非目标”和“目标”分析）。因此，这一建议的目的不仅是为了更换购买，同时还结合了一项重大创新，提供了新的分析选择。高分辨率的分析尤其证明了这一点，这种分析最近是不可能的，并且可以同时检测和识别（至少通过求和公式）大量化合物。这使得在物质鉴定方面的创新研究得以实现：化合物混合物的测定和鉴定，代谢物和降解途径的鉴定，高分辨率的非靶标分析。与现有系统相比，更高的灵敏度允许达到低数量级的检测极限。这将特别在与环境科学特别相关的复杂基质中实现（例如，植物和动物组织的提取物、灵魂和沉积物、废水、污泥和废物）。更广泛的质量范围提供了新的应用选择，从蛋白质分析的角度来看（蛋白质组学）。运维所需的工作量和预期的运行成本与应用部门的绩效框架相对应。

Several departments of the faculty VI „Regional and Environmental Sciences“ of the University Trier apply for a system for high-resolution liquid chromatography-mass spectrometry (LC-MS-QTOF). The system will (i) replace an older liquid chromatography-tandem mass spectrometry-system (in use since 2006) and (ii) provide new options for more selective and sensitive analytics, which especially enables screening analysis of complex mixtures of substances of anthropogenic as well as natural origin. The need for such analytics is justified by on-going and planned projects. Being the only analytical system of this kind at the University Trier it makes a powerful enrichment for the analytical equipment and will be cooperatively used within the group of departments and laboratory cluster, respectively, as is the case with the existing equipment. Environmental analysis using mass spectrometry is done in research and education since many years in the department of Soil Science (applicant) as well as in the co-applying departments and further departments of the faculty VI. The aforementioned liquid chromatography-tandem mass spectrometry-system provided very good analytical options at date of purchase that substantially fostered the acquisition of various funded projects. Due to its service life, the system is no more reliable; downtimes and repair times increased to an extent that conflicts with routine operation and use in time-limited projects.Therefore, we apply for a liquid chromatography-mass spectrometry-system that is capable for high-resolution determination of unknown compounds and can also be used for routine detection and quantification of known compounds („non-target“ and „target“ analysis). This proposal is, thus, aimed not only for a replacement purchase but at the same time combined with a significant innovation, offering new analytical options. This is especially justified by the high-resolution analysis that is recently not possible and allows to simultaneously detect and identify (at least by the sum formula) large numbers of compounds. This enables to realize innovative studies on substance identification: Determination and identification of compound mixtures, identification of metabolites and degradation pathways, high-resolution non-target analysis. The substantially higher sensitivity allows to reach limits of detection that are by magnitudes lower compared to the existing system. This will be achieved in particular in complex matrices that are especially relevant in environmental sciences (e.g., extracts of plants and animal tissues, souls and sediments, waste water, sludge and wastes). The much broader mass range offers new application options, perspectively up to the analysis of proteins (Proteomics). The required efforts for operation and maintenance as well as the expected running costs correspond to the performance framework of the applying departments.