Diagnosis of acute rejection using anti ICAM-1 and amlanti LFA-1 auhbahy in rat pancreatic transplantation

在本研究中，ICAM-1和LFA-1在胰腺移植后的胰液和胰腺移植物中的出现进行了研究。通过胰液细胞学检查，胰腺移植排斥反应可通过淋巴母细胞、淋巴细胞、单核细胞和巨噬细胞的增加来诊断。研究ICAM-1和LFA-1在体液中的出现方式对早期诊断急性排斥反应有一定的意义。然而，使用大鼠的胰腺移植难以达到可接受的结果。此外，发现难以获得大鼠的胰液。我们的模型是通过小口径管将胰液取出，但大鼠经常切断管。即使保存试管，也不能获得足够量的汁液。为了获得胰液，可能需要一个可以放置在皮下的端口。因此，我们未能研究大鼠胰液中包含的细胞。这种类型的研究在人类胰腺移植中是可能的。

In the present study, the appearance of ICAM-1 and LFA-1 in the pancreatic juice and pancreatic grafts are investigated following pancreatic transplantation. By pancreatic juice cytology, pancreatic graft rejection is diagnosed by the increase of lympho blasts, lymphocytes, monocytes and macrophages. It may be useful to investigate the mode of appearance of ICAM-1 and LFA-1 in the cells included in the juice for diagnosing acute rejection in the early stage. However, pancreatic transplantation using rats is difficult to achieve acceptable results. Furthermore, it was found to be difficult to obtain pancreatic juice in rats. By our model, the pancreatic juice was exteriorized via a small caliber tube, but rats cut the tubes frequently. Even if the tubes were preserved, sufficient amount of juice could not be obtained. In order to obtain pancreatic juice, a port, which can be placed subcutaneously, may be necessary. Thus we failed in investigating the cells included in the pancreatic juice in the rats. This type of research may be possible in human pancreatic transplantation.