Study on the abnormal expression of cytokine and growth factor genes in human malingnant gliomas.

这个项目已经产生了相当多的成果。我们发现恶性星形细胞瘤(胶质瘤)细胞产生白介素8(IL-8)，特别是以77位氨基酸形式(Tada M等，J Neuro-Onol 15：25)。我们还发现肿瘤坏死因子-α刺激胶质瘤细胞产生各种因子，如IL-6、IL-8、Mn-超氧化物歧化酶和IL-1(Sawamura Y等，J Neuro-Oncol15：197)。我们已报道胶质瘤细胞产生粒细胞-巨噬细胞集落刺激因子(Tawamura Y，等，Neurol Med Chir 33：603)、单核细胞趋化蛋白-1(Tawamura Y，等，Neurol Med Chir 33：603白介素1受体拮抗剂(Tada M等，神经免疫杂志50：187)。其中一名调查员(M.T.)综述了胶质瘤细胞产生的细胞因子(Tada M等人，J Neuro-Oncol17：261)，这些细胞因子和生长因子在脑胶质瘤患者的脑脊液中检测到，并被认为对疾病的诊断有帮助(Sawamura Y，等人)。神经免疫Res 8：204)。关于这些细胞因子和生长因子的受体，我们用RT-PCR方法分析发现，胶质瘤细胞表达IL-1受体I型，2型；p55，p75肿瘤坏死因子受体；干扰素α/β，伽马受体，GM-CSF受体，M-CSF受体，SCF受体，和IL-8受体(Tada M，等，神经外科杂志80：1063；Sawamura，等，Neurol Med Chir 35：567)。

This project has yielded a considerable amount of resutls. We found that malignant astrocytoma (glioma) cells produce interleukin-8 (IL-8), especially in 77-amino acid form (Tada M,et al., J Neuro-Oncol 15 : 25) Also we found that tumor necrosis factor-alpha stimulates glioma cells to produce various factors such as IL-6, IL-8, Mn-superoxide dismutase, and IL-1 (Sawamura Y,et al., J Neuro-Oncol 15 : 197) We have reported that glioma cells produce granulocyte-macrophage colony-stimutating factor (Sawamura Y,et al., Neurol Med Chir 33 : 603), monocyte chemoattractant protein-1 (Tada M,et al., Int J Cancer 58 : 240), interleukin-1 receptor antagonist (Tada M,et al., J Neuroimmunol 50 : 187). One of the investigator (M.T.) reviewed the cytokine production by glioma cells (Tada M,et al., J Neuro-Oncol 17 : 261) These cytokines and growth factors are detected in cerebrospinal fluid of patients with glioma, and considered to be useful for diagnosis of the disease (Sawamura Y,et al. Neuroimmunol Res 8 : 204). Concerning receptors for these cytokines and growth factors, we analyzed with RT-PCR method and found that glioma cells express IL-1 receptor type I,type2 ; p55, p75 TNF receptor ; interferon alpha/beta, gamma receptor, GM-CSF receptor, M-CSF receptor, SCF receptor, and IL-8 receptor (Tada M,et al., J Neurosurg 80 : 1063 ; Sawamura Y,et al., Neurol Med Chir 35 : 567)