Immunodetection and immunotherapy for metastatic testicular germ cell tumors using antiplacental alkaline phosphatase monoclonal antibody

我们研究了胎盘碱性磷酸酶（PLAP）作为乳腺癌肿瘤标志物的意义，并评估了PLAP作为免疫检测和免疫治疗的靶向抗原的潜力。监测血清PLAP可能有价值，因为该标志物的波动提供了疾病状态和预后的信息。在裸鼠模型中证实了抗PLAP MAb对产生PLAP的异种移植物的特异性定位。在睾丸肿瘤及其淋巴结转移的SCID小鼠模型中，MAb在淋巴结转移中的定位比在睾丸肿瘤中的定位更有效，这表明抗PLAP MAb用于放射免疫治疗（RIT）的潜在用途。然而，RIT对转移灶的疗效仍有待澄清，因为在用I-131标记的抗PLAP单抗处理的小鼠模型中不太可能发生肿瘤细胞的完全消除。

We investigated significance of placental alkaline phosphase (PLAP) as a tumor marker for seminoma, and assessed a potential of PLAP as a targeting antigen for immunodetection and immunotherapy using anti-PLAP MAb. The monitoring of serum PLAP might be of value, as fluctuations in this marker provide information adout disease status and prognosis. Specific localization of anti-PLAP MAb to PLAP-producing xenografts was confirmed in a nude mouse model. In a SCID mouse model of testicular tumor and its lymph node metastases, localization of the MAb in lymph node metastases was more efficient than localization in testis tumors, suggesting the potential use of anti-PLAP MAb for radioimmunotherapy (RIT) on small metastatic foci of seminoma. However, efficacy of RIT on the metastaitc foci remains to be clarified since complete elimination of tumorcells was unlikely to occur in the mouse model treated with I-131 labeled anti-PLAP MAb.