Development of New Sensing Methods for Bioactive Ions and Molecules by Mimicking Homeostasis in Biological Membranes

1)利用从牛脑中分离纯化的钙调蛋白和一种合成肽M13研制了一种光学传感器。用表面等离子体共振法研究了Ca~(2+)~(2+)/CaM_(13)/CaM_(13)三元络合物的形成。2)以拉沙洛西德为载体，构建了以液膜为载体的多巴胺主动转运体系。利用活性转运系统，提高了多巴胺在NM水平的浓度。3)成功地将合成的受体直接掺入折叠法制备的平面双层脂膜中。利用这些受体修饰的BLMS设计了新的电位传感系统。

New Sensing Membranes based on the principle of homeostasis have been developed.1) An optical sensor has been described by using calmodulin (isolated and purified from calf brain) and a synthetic peptide M13. Formation of a ternary complex between Ca^<2+>/calmodulin and M13 was monitored by using the surface plasomon resonance method. A highly sensitive and selective detection of several tens nM Ca^<2+> ions was demonstrated.2) An active transport system for dopamine was constructed by using lasalocid as a carrier dissolved in liquid membranes. Enhancement of concetratio of dopamine at nM levels was achived by using the active transport system.3) Direct incorporation of synthetic receptors into planar bilayer lipid membranes formed by the folding method was successfully achieved. New potentiometric sensing systems were designed by using these receptor-incorporated BLMs.