Effects of oxygenation during initial perfusion of the liver through the double-balloon cathter on the graft viability

脑死亡状态下不可避免地会出现全身性低血压，除非得到适当的治疗，否则这种低血压的不稳定性可能会导致肝循环减少，最终导致移植物存活率降低。本研究采用双球囊导管灌注脑死亡兔模型，观察了双球囊导管灌注氧合对单纯低温保存后肝脏能量代谢和形态完整性的影响。在该模型中，诱导低全身血压。脑死亡状态下肝脏能量变化和血流动力学较脑死亡前明显降低。这些结果提示移植物活性丧失。在简单的低温保存过程中，在肝脏能量代谢方面，使用双球囊导管进行初始灌注期间的氧合可有效地维持活力。电镜观察也显示了氧合对肝细胞线粒体变性过程进展的预防作用。

Instability of the systemic hypotension, that is inevitably encountered inthe state of brain death unless it is appropriately treated, may cause decrease in the hepaticcirculation and eventually result in reduction of the graft viability. We assessed effects of oxygenation during initial perfusion through double balloon cathter in brain-dead rabbits on changes in the energy metabolism and morphological integraity of the liver following a simple hypothermic storage.A brain death model was successfully prepared in rabbit by intracranially inserting a balloon. In this model, the low systemic blood pressure were induced. Under the state of brain death, thehepatic enegy change and the hemodynamics were significantly reduced compared with those in the condition of pre-brain death. These findings were suggested the loss of graft viability. Oxygenation during initial perfusion using the double balloon catheter of the liver was effective in maintaining the viability, in terms of hepatic nergy metabolism, during a simple hypotherimic preservation. Electron microscopic observation also showed preventive effects of oxygenation on progression of the degeneration process of the mitochondria of hepatocytes.