Integrated confocal luminescence spectrometer with spatiotemporal resolution and multiphoton excitation

功能材料的光物理研究对于深入理解亚分子和超分子水平上的发光激发态性质起着基础性的作用。对于正在进行的和未来的项目在地方，国家和国际一级，国家的最先进的光谱和时间分辨发光分析技术需要与高空间和时间分辨率的共焦光学显微镜与多光子激发能力（包括发光寿命成像显微镜）相结合。这将构成一个独特的组合，目前还没有这种特定的形式。由于凝聚相和界面的高度散射性质，在不同温度下以光谱、空间和时间分辨率测量溶液、均匀基质、纤维、单层或多层、薄膜、无定形粉末、晶体、纳米或微米颗粒中单体或聚集分子发射体的发光提出了特殊的挑战。当需要在培养基、细菌、生物膜、细胞器、细胞和组织中以光谱、空间和时间分辨率评估光敏剂、荧光或磷光探针的激发态性质时，这也适用。特别是，与matrixial，细菌和真核生物（宿主细胞或原生动物寄生虫）组件的特定相互作用需要进行原位监测。此外，双光子吸收截面将确定在均匀的阶段。这些挑战将通过所要求的仪器来克服，这也将使双光子激发性的评估能够达到生物成像和光疗的深层结构。

The photophysical investigation of functional materials plays a fundamental role towards an in-depth understanding of luminescent excited state properties at sub- and supramolecular level. For ongoing and future projects at local, national and international level, state of the art spectrally and temporally resolved luminescence analysis techniques need to be combined with the high spatial and temporal resolution of confocal optical microscopy with multiphoton excitation capabilities (including luminescence lifetime imaging microscopy). This will constitute a unique combination that is presently not available in this particular form. Measuring the luminescence of monomeric or aggregated molecular emitters in solutions, homogeneous matrices, fibres, mono- or multilayers, thin films, amorphous powders, crystals, nano- or microparticles with spectral, spatial and temporal resolution at variable temperatures poses a particular challenge due to the highly scattering nature of condensed phases and interfaces. This also applies when the excited state properties of photosensitizers, fluorescent or phosphorescent probes need to be assessed with spectral, spatial and temporal resolution in culture media, bacteria, biofilms, organelles, cells and tissues. In particular, the specific interactions with matrixial, bacterial and eukaryotic (hosts cells or protozoic parasites) components need to be monitored in situ. Moreover, two-photon absorption cross-sections will be determined in homogeneous phases. These challenges will be overcome with the requested instrumentation, which will also enable the assessment of two-photon excitability to reach deep lying structures for bioimaging and phototherapy.