The electrical property, chemoreceptivity and synaptic transmission of autonomic neurons

1.交感神经节前神经元(CAT)的体外研究：1.对交感神经节前神经元(CAT)的静息和活性膜特性、离子电导、化学感受性、快慢突触电位及其化学递质以及去甲肾上腺素的调节作用进行了研究。边缘神经元和中枢单胺能神经元的体外研究在这些神经元中，海马区锥体神经元受到了低氧的影响。低氧引起双相(超极化-去极化)膜电位改变。这些变化可分别归因于钙离子大量进入细胞质而引起的原生和继发变化。多巴胺对伏核神经元作用的研究表明，多巴胺通过D-1受体产生超极化，通过D-2受体产生去极化。交感神经节(蛙)内肽能传递的研究LHRH和乙酰胆碱在神经节前C纤维终末共存的释放方式以及LHRH介导的慢突触电位的细胞内调控系统。肠黏膜下神经丛神经元(豚鼠)的研究已阐明慢EPSP和慢IPSP的电发生机制、化学递质和细胞内信号转导。心内(副交感)神经节细胞(豚鼠)的研究分析与烟碱受体激活有关的鼻电流揭示了两种不同开放时间的受体-离子载体复合体。

This team has accomplished the following studies in the past three years beginning 1985:1. In-vitro study of sympathetic preganglionic neurons (cat); the resting and active membrane characteristics, ionic conductances, chemoreceptivities, fast and slow synaptic potentials and their chemical transmitters, and the modulatory action of noradrenaline has been clarified.2. In-vitro study of limbic neurons and central monoaminergic neurons; among these neurons, hippocampal pyramidal neurons have been subjected to the investigation of the effect of hypoxia. Hypoxia caused biphasic (hyper-polarizing-depolarizing) memrane potential changes. These changes can be attributed, respectively, to the primary and the secondary alteration resulted from an excessive entry of Ca ions into the cytoplasm. The study of dopamine action on neurons of the nucleus accumbens revealed that dopamine produces hyperpolarization via D-1 receptors and depolarization via D-2 receptors.3. Study of peptidergic transmission in sympathetic ganglia (frog) ; the mode of liberation of LHRH and acetylcholine which coexist in the preganglionic C nerve fiber terminals and the intracellular modulating system for the LHRH-mediating slow synaptic potential have been characterized.4. Study of enteric submucous plexus neurons (guinea-pig) ; electrogeneses, chemical transmitter substances and intracellular signal transductions for the slow EPSP and slow IPSP have been clarified.5. Study of intracardiac (parasympathetic) ganglion cells (guinea-pig) ; analysis of the nose currents associated withe the activation of nicotinic receptors revealed two kinds of receptor-ionophore complexes which characteristically differ in open time.