Optogenetic membrane manipulation of protein targeting to mitochondria and plastids (A13)

我们的数据表明，一些蛋白在有氧条件下靶向莱茵衣原体的质体，在厌氧条件下（以及线粒体δ psi的损失）双靶向线粒体和质体。我们计划生成光遗传可控的细胞器，包括膜集成视蛋白来操纵δ psi，以及通过光遗传开关调节膜结合受体平台和磷酸酶。我们的工作将(i)揭示靶向序列与δ psi和受体介导的膜身份之间的相互作用，确保正确的靶向，以及（ii）实现新的光遗传学工具来理解和控制C. reinhardtii的细胞过程。

Our data demonstrate that some proteins targeted to the plastid under aerobic conditions in Chlamydo-monas reinhardtii, are dual-targeted to mitochondrion and plastid under anaerobic conditions (and loss of mitochondrial delta psi). We plan to generate optogenetically controllable organelles, both in terms of membrane-integrated opsins to manipulate delta psi, as well as modulating membrane-bound receptor platforms and phosphatases through optogenetic switches. Our work will (i) shed new light on the interplay of targeting sequences and delta psi - and receptor-mediated membrane identity that secures correct targeting, and (ii) implement new optogenetic tools for understanding and controlling cellular processes in C. reinhardtii.