Biochemical and Histological Studies on the Possible Mechanism of Mouse Muscular Hypotrophy

同类型（dy/dy）和异类型（dy/？）男性C57 BL/6 J-dy老鼠（5-6 wks和8-9 wks old）在生物化学和历史上都被用于非运动肌肉催眠的可能机制。Whole hindlimb muscles were minced with scissors， and homogenized with 25 mM phosphate buffer（pH7. 4） at 0 C。同源物分为缓冲区溶解度（F-1）、1.2 M KCl溶解度（F-2）、1.2 M KCl溶解度（F-3）、核糖核酸（RNA）、脱氧核糖核酸（DNA）和蛋白质。嵌入石蜡M的串行交叉部分（10<mu>米厚）。Gasrocnemius和M. Soleus被保留在Hematoxylin和Eosin中。每个单元区域的Nucleus和muscle Fiber的数字（ &lt;1 mm ^2&gt; ）和muscle Fiber的交叉部分被确定。（1）; [DNA]和[RNA]在dy/dy中，老鼠比在dy/dy中高出2.5-2.8次和1.5-1.8次？老鼠，尊敬的。每一种核酸浓度的明显分解是由muscular hypotrophy引起的。（二）; [蛋白质]的肌肉组织在迪 ... More “老鼠比那个小1.22次？”mice.（三）;迪/迪估计的[蛋白质]/DNA和肌肉重量/DNA中的明显肌肉细胞体积比迪7.9次和3.0-3.4次低。老鼠，尊敬的。（四）;每一个肌肉片段中蛋白质含量的降解顺序是F-2&gt; F-3&gt; F-1，表明蛋白质含量的降解与每个片段是不确定的。（五）。每个片段中的麝香蛋白的电形态模式都被麝香蛋白质的流变学改变了，这表明这种催眠学改变了麝香蛋白质的分形组成比率。（六）。“在白天的核/老鼠的数字比在白天的数字大1.9次？”mice.（七）。中央核的数量与核的总数量在dy/dy老鼠的比率比在dy/dy大9.5次？mice.（八）。在dy/dy/dy/dy上安装口罩的区域频率分布从正常分布配置文件中？mice.平均面积（0.0049 &lt;mm^2&gt; ）在dy/dy的交叉部位比在那个（0.0072 &lt;mm^2&gt; ）小0.68次。mice.（九）。Muscle hypotrophy的可能机制在细节中被讨论。Less（低）

The homo-type(dy/dy) and hetero-type(dy/?) of male C57BL/6J-dy mice(5-6 wks and 8-9 wks old) were used to elucidate biochemically and histologically the possible mechanism of non-exercise muscular hypotrophy. Whole hindlimb muscles were minced with scissors, and homogenized with 25mM phosphate buffer(pH 7.4) at 0゜C. The homogenate was fractionated into buffer-soluble(F-1), 1.2M KCl-soluble(F-2), 1.2M KCl-insoluble(F-3), ribonucleic acid(RNA), deoxyribonucleic acid(DNA) and protein. Serial cross section(10 <mu> m thick) of paraffin embedded M. gasrocnemius and M. soleus were stained with hematoxylin and eosin. The numbers of nucleus and muscle fiber per unit area( <1mm^2> ) and the area of cross section of muscle fiber were determined. 1). [DNA] and [RNA] of hindlimb muscles in dy/dy mice was 2.5-2.8 times and 1.5-1.8 times higher than that in dy/? mice, respectively. Each nucleic acid concentration decreased significantly by muscular hypotrophy. 2). [protein] of the muscle tissue in dy … More /dy mice was 1.22 times lower than that in dy/? mice. 3). Apparent muscle cell volume in dy/dy mice estimated from [protein]/DNA and muscle weight/DNA, was 7.9 times and 3.0-3.4 times lower than those in dy? mice, respectively. 4). The order of decrease of protein contents in each muscle fraction by muscle hypotrophy was F-2>F-3>F-1, indicating that the decrease of protein content is not identical with each fraction. 5). Electrophoretic pattern of muscle proteins in each fraction was changed drastically by muscle hypotrophy, indicating that the hypotrophy changes the fractional composition ratio of muscle proteins. 6). The numbers of nucleus in dy/dy mice was 1.9 times larger than that in dy/? mice. 7). The ratio of the number of centronucleus to total numbers of nucleus in dy/dy mice was 9.5 times larger than that in dy/? mice. 8). Area frequency distribution of muscles in dy/dy mice deviated from the normal distribution profile in dy/? mice. The mean area(0.0049 <mm^2> ) of the cross section in dy/dy mice was 0.68 times smaller than that(0.0072 <mm^2> ) in dy/? mice. 9). The possible mechanism of muscle hypotrophy was discussed in detail. Less