Studies on pathophysiology and treatment of cerebral hypoxia with special reference to blood brain barrier

1.研究了甲基强的松龙、乌司他丁、维生素E和辅酶Q对颈动脉注射高渗甘露醇所致大鼠脑膜稳定性的影响，发现甲基强的松龙和乌司他丁对伊文思蓝有明显的抑制作用。但维生素E和辅酶Q2无显著差异。应用钙通道阻滞剂氟桂利嗪，测定大鼠吸氧后及恢复期纹状体中儿茶酚胺及其代谢产物(多巴胺、HVA、DOPAC、去甲肾上腺素)的浓度，研究其对儿茶酚胺波动的影响。吸入5%氧气后，多巴胺含量增加。DOPAC和去甲肾上腺素降低，HVA无变化。低氧20min后，再吸空气2小时后，血儿茶酚胺含量恢复至对照组水平。Con…中的更改在缺氧和随后的恢复期，更多的帐篷似乎更低。用电子显微镜和X射线显微镜观察了吸入5%氧气和夹闭双侧颈总动脉15min后大鼠锥体神经细胞的形态变化和钙蓄积。为了研究尼卡地平对脑缺氧的保护作用，在低氧事件后采用相同的技术。吸氧5%组，胞核可见染色质钳制，但神经元内未检测到钙离子。颈动脉夹闭组吸氧5%，线粒体肿胀，胞核严重改变，线粒体内可见钙离子。在低氧和低氧夹闭颈动脉下给予尼卡地平后，发现了相似的形态变化。因此，在本实验中，尼卡地平对缺血缺氧没有明显的保护作用。较少

1. The beneficial effect of methylpredonisolone, urinastatin, vitamin E and coenzyne Q on membrane stability was investigated in rat brains induced by an infusion of hypertonic mannitol solution via the carotid artery.It was found the pretreatment of methylpredonisolone and urinastatin significantly inhibited Evans blue strainly. But no significant difference was observed by vitamin E and coenzyne Q.2. The concentrations of catecolamins and their metabolites (dopamine, HVA, DOPAC, norepinephrine) in striatum was measured during 5% oxygen inhalation and subsequent recovery period in rats, Flunarizine, a calcium channel blocker, was administered and its effect on catecholamine fluctuations was studied. Dopamine increased with 5% oxygen inhalation. DOPAC and norepinephrine decreased and no changes were seen in HVA. After 20 min of hypoxia, catecholamine contents returned to the control levels in another 2 hours of air breathing.When flunarizine was administered before hand. Changes in con … More tent appeared to be lower during hypoxia and subsequent recovery period.3. The morphological changes and calcium accumulation were studied in rat pyramidal nerve cell which had undergone 5% oxygen inhalation and bilateral carotid artery clampings for 15 minutes, using electron and X-ray microanalytical microscopes. To study the protective effects of nicardipine on brain hypoxia, the same techniques were used after hypoxic event. In the 5% oxygen inhalated group, the chromatinclamping in the nucleus was observed, but calcium ions could not be detected in the neuron. In the carotid clamping group under 5% oxygen inhalation, severe nuclear change and mitochondrial swelling were observed and calcium ions were detected in the mitochondrion. After administration of nicardipine under hypoxia and under carotid clamping with hypoxia, similar morphological changes were recognized. It is, therefore, concluded that notable protective effects of nicardipine on ischemic hypoxia were not obtained in this morphological experiment. Less