A Study of Cellular Communication on Malignant Progression in Urogenital Tumors

用染料传递法研究人泌尿生殖道上皮细胞间通讯(IC)。1)在移行细胞癌(TCC)细胞中，分化和未分化的TCC细胞分别呈高IC和低IC。不同TCC细胞的不同组合之间存在高IC或低IC的现象。成纤维细胞呈明显的IC，无TCC细胞。2)在前列腺上皮中，前列腺癌细胞间的IC程度低于良性前列腺疾病。从骨转移灶(PC-3)和淋巴结培养的前列腺癌细胞的IC非常有限。良性前列腺上皮与PC-3之间几乎没有免疫反应。3)睾酮对TCC细胞系IC有抑制作用。这种抑制是时间和剂量依赖的，在剥夺睾酮后迅速恢复。相反，雌二醇-17和皮质醇都不能抑制这些TCC细胞的IC。4)为了检测尿路上皮细胞的恶变，对细胞角化和细胞表面抗原进行了研究。(1)正常尿路上皮细胞角蛋白纤维细长、笔直、直径恒定，均匀分布于细胞质中。在TCC细胞中，它们直径不同，蜿蜒穿透，在细胞质中分布不均。5)3株抗TCC细胞株(JTC-30)的单抗与A血型抗原有关，但与抗A型单抗的反应模式不同。这些抗JTC-30单抗识别A血型相关表位(S)，该表位在恶变过程中是不稳定的。

Intercellular communication (IC) was investigated by dye transfer method in the human urogenital epithelia. 1) In transitional cell carcinoma (TCC) cells, high and low IC was observed in differenciated and undifferenciated TCCs, respectively. High or lack of IC was observed between various combination of different TCC cells. Fibroblasts showed apparent IC with none of TCC cells.2) In prostatic epithelium, the extent of IC among prostatic carcinoma cells was lower than that of benign prostatic diseases. IC of prostatic carcinoma cells cultured from metastatic lesions of bone (PC-3) and lymph nodes was very limited. IC between benign prostatic epithelium and PC-3 was almost absent. No IC was present between prostatic epithelium and interstitial cells.3) Testosterone inhibites IC of TCC cell lines. This inhibition was time- and dose-dependent and recovered rapidly after deprivation of testosterone. In contrast, neither estradiol-17 nor cortisol inhibites IC of these TCC cells. IC of fibroblasts was not influenced by testosterone.4) To detect malignant transformation of the urothelium, cytokeration and cell surface antigens were investigated. (1) In normal urothelium, keratin fibers are thin, straight, constant in diameter and are distributed evenly in the cytoplasm. In TCC cells, they vary in diameter, wind their ways through and are distributed unevenly in the cytoplasm. The changes of ketatin fibers in bladder carcinoma cells appeared to be parallel to the grade of malignancy.5) Three monoclonal antibodies (MoAb) generated against a TCC cell line (JTC-30) appeared to be related to blood group A antigen but have different reaction patterns with anti-blood group A MoAb. These anti-JTC-30 MoAbs recognize a blood group A related epitope(s), which is unstable in the process of malignant degeneration.