confocal laserscanning microskope including FLIM/FCS

神经元的生长受多种因素的调控。例如，细胞外侧富含受体活性磷脂。这些磷脂能够与g偶联受体结合，从而调节多种细胞过程，如增殖、迁移和细胞分化，并可作为神经元的排斥或吸引因子。溶血磷脂（LPA）等磷脂以相应的浓度存在于大脑中，并作用于神经突。直到最近，只有一类lpa修饰蛋白，脂质磷酸磷酸酶（LPPs）被发现。这些蛋白可以通过LPA去磷酸化调节细胞外侧LPA水平。2003年，我们发现了一类新的脑特异性lpa修饰蛋白，即可塑性相关基因（PRGs）。到目前为止，已经确定并克隆了五个不同的成员。所有这些都在大脑发育过程中动态表达，并通过改变LPA参与再生过程。基于这些发现，我目前的研究重点是确定磷脂在大脑中的功能相关性以及它们如何被控制。因此，我们能够确定一种新的轴突生长机制。此外，我们关于磷脂的功能相关性的结果表明，它们参与神经元传递过程。这一机制必须在未来得到澄清。

Neuronal outgrowth is regulated by different factors. The extracellular side, for example, is rich in receptor-active phospholipids. These phospholipids are able to bind to G-coupled receptors and thereby regulate multiple cellular processes such as proliferation, migration, and cell differentiation, and can act as repulsive or attractive factors on neurons. Phospholipids such as the lysophospholipids (LPA) are located in the brain in relevant concentrations and act on neurites. Until recently, only one LPA-modifying protein class, the lipid phosphate phosphatases (LPPs), was known. These proteins can regulate the LPA level on the extracellular side by LPA dephosphorylation. In 2003, we found a novel class of brain-specific LPA-modifying proteins, the plasticity-related genes (PRGs). So far, five different members have been identified and cloned. All are dynamically expressed during brain development and involved in regeneration processes by modifying LPA. Based on these findings, I am currently focusing my research on identifying the functional relevance of phospholipids in the brain and how they are controlled. Thus, we were able to identify a novel mechanism of axonal outgrowth. Furthermore, our results on the functional relevance of phospholipids suggest that they are involved in neuronal transmission processes. This mechanism has to be clarified in the future.