The effects of Actinomyces viscosus and Bacteroides gingivalis on bone resorption

许多研究表明，在青少年牙周炎中，口腔细菌侵入牙周组织，导致牙周破坏。但在成人牙周炎中，这些发现尚未得到澄清。本研究旨在了解成人牙周炎时细菌侵袭与牙槽骨吸收的关系。选择从人牙周袋中分离的粘性放线菌和牙龈拟杆菌。使用3周龄和9周龄的Wister品系大鼠。在接种细菌之前，在下颌第一磨牙上进行丝线结扎。将2株细菌接种于大鼠口腔内42天。接种后42天观察到轻微的牙龈炎症。然后将这些大鼠处死并制作组织切片。组织切片用苏木精-伊红和抗粘性放线菌血清或抗牙龈拟杆菌血清染色，并结合荧光技术。苏木精-伊红染色切片可见牙周袋形成，牙龈轻度炎症，结合上皮向根尖移动，牙槽骨吸收明显。在荧光染色切片上，粘性放线菌在结合上皮中可见，而在结缔组织中未见;牙龈拟杆菌在结合上皮与口腔上皮交界处的上皮中可见，而在结缔组织中未见。认为在牙槽骨吸收的初期，这两种细菌并不直接参与骨吸收。

Many reports suggested that in juvenile periodontitis, oral bacteria invade into periodontal tissue and cause periodontal breakdown. But in adult periodontitis, these findings have not been clarified. The purpose of investigation is to know the relationship between bacerial invasion and alveolar bone resorption in adult periodontitis. Actinomyces viscosus and bacteroides gingivalis isolated from human periodontal pocket were selected. Wister strain rats aged 3 weeks and 9 weeks were used. Before the inoculation of the bacteria, silk ligature was made on the mandibular lst molar. The 2 strain of bacteria were inoculated into the rat oral cavity for 42 days. Slight gingival inflammation was observed on 42 days after the inoculation. Then these rats were sacrefied and the histological sections were made. Histological sections were stained with hematoxylin-eosin and with anti Actinomyces viscosus sera or anti Bacteroides gingivalis sera associated with fluorescent rechnique. On the hematoxylin-eosin stained sections, periodontal pocket formation with slight gingival inflammation and the apical migration of junctional epithelium, and sight alveolar bone resorption were observed. On the section treated with fluorescent technique, Actinomyces viscosus was found into the junctional epithelium but not in connective tissue and Bacteroides gingivalis was observed in the epithelium where junctional epithelium where junctional epithelium and oral epithelium junction but not in connective tisue. From these findings, it is considered that the initial stage of alveolar bone resorption, the 2 bacteria do not perticipate directly in bone resorption.