Experimental and clinical studies on oncogenesis of salivary glands.

对我院手术切除的70例唾液腺肿瘤进行免疫组织化学染色。s - 100a免疫染色在多形性腺瘤中呈阳性。多形性腺瘤的二重奏样结构保留了乳铁蛋白的产生和分泌成分的功能，并与功能分化相适应。这一结果支持了Regezi关于唾液腺肿瘤起源的双细胞理论。对裸鼠皮下移植的低级别分化粘液表皮样瘤进行了这些组织学特征的研究。我们认为黏液表皮样瘤的黏液分泌细胞可由表皮样细胞和中间细胞分化或转化。为了阐明唾液腺细胞在出生后的分化，对大鼠颌下腺进行了超微结构和形态计量学检查。在唾液腺发育过程中，大鼠颌下腺终小管细胞逐渐减少，出生后约6周消失，此期间腺泡细胞大量增加。超微结构显示唾液腺末端小管细胞不转化为腺泡细胞。此外，断奶较晚导致乳腺发育明显迟缓。用肌动蛋白（actin）、nbd -阴茎酸素（NBD-phallacidin）荧光探针观察了大鼠舌下腺肌上皮细胞的形态分化。新生儿时，腺泡周围出现弥漫性荧光细胞，代表早期的肌上皮细胞。出生后3至4天，肌动蛋白染色集中在平行于细胞突起长轴排列的链上。肌上皮细胞在出生后10天进一步发育，其突起的数量和厚度增加。出生后第60天发现发育完全的肌上皮细胞。这些是星状的，包围着单个的腺泡。

70 cases of salivary gland tumors removed surgically in our hospital were stained immunohistochemically. Positive immunostaining for S-100 a was observed in pleomorphic adenoma specifically. Duet-like structure in pleomorphic adenoma retains the function of production of lactoferrin and secretory component in accordance with functional differentiation. This results supported Regezi's bicellular theory on the origin of salivary gland tumor.The low grade differentiated mucoepidermoid tumor, transplanted subcutaneously to nude mouse, was studied on these histophathological feature. We concluded that mucous secreting cells of mucoepidermoid tumor would be differentiated or transformed from the epidermoid cells and the intermediated cells.To elucidate the postnatal differentiation of salivary gland cells, rat submandibular gland were examined ultrastructurally and morphometrically. During the development of salivary glands, terminal tubule cells of rat submandibular gland decreased gradually and disappeared almost 6 weeks after birth, acinar cells increased greatly in this period. Ultrastructural finding suggested that terminal tubule cells of salivary gland do not transform into acinar cells. Furthermore, late weaning resulted in marked developmental delay of the gland.Morphological dfferentiation of myoepitherlial cells of rat sublingual gland was examined by using the fluorescent probe for actin, NBD-phallacidin. In newborn, cells with diffuse fluoresecence occurred around the acini, representing incipient myoepithelial cells. Between 3 and 4 days after birth, actin staining was concentrated in strands which were arranged parallel to the long axis of the cell processes. Myoepithelial cells had developed further by 10 days after birth with an increased number and thickness of their processes. Fully developed myoepithelial cells were found on day 60 after birth. These were stellate and encompassed individual acini.