Detection of Cytomegalovirus Gene

巨细胞病毒基因检测

• 批准号: 08672317
• 负责人: TANIOKA Hiroaki
• 金额: $ 1.41万
• 依托单位: EHIME UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672317/
• 关键词: Cytomagalovirus; Polymerase chain reaction method; Salivary gland; Peripheral blood mononuclear cells; 7型ヘルペスウイルス; nested PCR; 難治性口腔粘膜病変; 単純ヘルペスウイルス1型; 単純ヘルペスウイルスI型; in situ PCR

According to recent studies, it seems that the host cells carrying cytomegalovirus (CMV) are not salivary gland cells but are lymphocytes and monocytes in the peripheral blood. However, this has not yet been clearly established because it is technically difficult to detect CMV-DNA in lymphocytes or monocytes from healthy CMV carriers by PCR methods.In this study, the nested PCR Method was employed for the detection of CMV-DNA in salivary glands obtained during surgery or autopsy and in peripheral blood mononuclear cells from healthy volunteers to figure out the target cells of the latent infection.CMV-DNA was detected in 28%(14/50) of the salivary glands and in 85.2 %(46/54) of the peripheral blood mononuclear cells. It is concluded that the target cells of CMV may be peripheral blood mononuclear cells.The PCR methods by synthetic oligonucleotide primer pairs are so sensitive that it is useful to either protection against transfusion-transmitted CMV infection or rapid detection and grasp of CMV infection in immunocompromised patients induced by solid organ or bone marrow transplants. These are current clinical matters.

根据最近的研究，携带巨细胞病毒(CMV)的宿主细胞似乎不是唾液腺细胞，而是外周血中的淋巴细胞和单核细胞。本研究采用套式聚合酶链式反应方法对手术或尸检中采集的唾液腺和健康志愿者外周血单个核细胞中的CMV-DNA进行检测，以确定潜在感染的靶细胞。28%(14/50)的唾液腺和85.2%(46/54)的外周血单核细胞中检测到CMV-DNA。结论：CMV的靶细胞可能是外周血单个核细胞，合成的寡核苷酸引物聚合酶链式反应方法灵敏度高，可用于预防输血传播的CMV感染或快速检测和掌握实体器官或骨髓移植后免疫功能低下患者的CMV感染。这些都是当前的临床问题。