Detection of Cytomegalovirus Gene

近年来的研究表明，巨细胞病毒（CMV）的宿主细胞不是唾液腺细胞，而是外周血中的淋巴细胞和单核细胞。然而，这一点尚未明确确立，因为通过PCR方法检测来自健康CMV携带者的淋巴细胞或单核细胞中的CMV-DNA在技术上是困难的。采用巢式PCR方法检测手术或尸检中的唾液腺组织和健康志愿者外周血单个核细胞中的CMV-DNA，以确定潜伏感染的靶细胞。28%（14/50）的唾液腺和85.2%（46/54）的外周血单个核细胞中检测到DNA。结论：CMV的靶细胞可能是外周血单个核细胞，合成的寡核苷酸引物PCR方法敏感性高，可用于预防输血传播的CMV感染，也可用于实体器官或骨髓移植后免疫功能低下患者CMV感染的快速检测和掌握。这些都是当前的临床问题。

According to recent studies, it seems that the host cells carrying cytomegalovirus (CMV) are not salivary gland cells but are lymphocytes and monocytes in the peripheral blood. However, this has not yet been clearly established because it is technically difficult to detect CMV-DNA in lymphocytes or monocytes from healthy CMV carriers by PCR methods.In this study, the nested PCR Method was employed for the detection of CMV-DNA in salivary glands obtained during surgery or autopsy and in peripheral blood mononuclear cells from healthy volunteers to figure out the target cells of the latent infection.CMV-DNA was detected in 28%(14/50) of the salivary glands and in 85.2 %(46/54) of the peripheral blood mononuclear cells. It is concluded that the target cells of CMV may be peripheral blood mononuclear cells.The PCR methods by synthetic oligonucleotide primer pairs are so sensitive that it is useful to either protection against transfusion-transmitted CMV infection or rapid detection and grasp of CMV infection in immunocompromised patients induced by solid organ or bone marrow transplants. These are current clinical matters.